Application
Western blot
Sample
Chinese hamster Cell lysate - nuclear (Cell line CHO)
Gel Running Conditions
Non-reduced Denaturing (gel 4-12% Bis Acrylamide)
Loading amount
20 µg
Specification
Cell line CHO
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Donkey anti rabbit HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Clarity western ECL substrate
Exposure
20 second(s)
Bands
Specific: 155 kDa
Positive control
We used glial cell line for positive control
Additional data
Additional Notes
This antibody don't work in CHO cell line
Abcam user community
Verified customer
Submitted Apr 27 2017