Overview

  • Product name

    Anti-NCAM1 antibody [EP2566(2)]
    See all NCAM1 primary antibodies
  • Description

    Rabbit monoclonal [EP2566(2)] to NCAM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NCAM1. The exact sequence is proprietary.

  • Positive control

    • Human cerebellum, oligodendroglioma and SH-SY5Y lysates; Human glioma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 94 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF or IP.
  • Target

    • Function

      This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
    • Sequence similarities

      Contains 2 fibronectin type-III domains.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization

      Secreted and Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
      • antigen recognized by monoclonal antibody 5.1H11 antibody
      • CD56 antibody
      • cell adhesion molecule, neural, 1 antibody
      • MSK 39 antibody
      • MSK39 antibody
      • N-CAM-1 antibody
      • NCAM 1 antibody
      • NCAM antibody
      • NCAM C antibody
      • NCAM-1 antibody
      • NCAM1 antibody
      • NCAM1_HUMAN antibody
      • NCAMC antibody
      • Neural cell adhesion molecule 1 antibody
      • Neural cell adhesion molecule NCAM antibody
      • OTTHUMP00000235666 antibody
      see all

    Images

    • All lanes : Anti-NCAM1 antibody [EP2566(2)] (ab137086) at 1/10000 dilution

      Lane 1 : Human cerebellum lysate
      Lane 2 : Human oligodendroglioma lysate
      Lane 3 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 94 kDa

    • Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling NCAM with ab137086 at 1/250 dilution.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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