Recombinant Anti-NCAM1 antibody [EPR2566] (ab133345)
Rabbit recombinant monoclonal NCAM1 antibody [EPR2566]. Validated in WB, IHC, ICC/IF and tested in Human. Cited in 4 publication(s).
- Datasheet
- References (5)
- Protocols
Overview
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Product name
Anti-NCAM1 antibody [EPR2566]
See all NCAM1 primary antibodies -
Description
Rabbit monoclonal [EPR2566] to NCAM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NCAM1 aa 250-350 (extracellular). The exact sequence is proprietary.
Database link: P13591 -
Positive control
- SH-SY5Y and Human fetal brain cell lysates; Human glioma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR2566 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab133345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 140-180 kDa (predicted molecular weight: 95 kDa). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/50 - 1/100. |
Target
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Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
Sequence similarities
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4684 Human
- Omim: 116930 Human
- SwissProt: P13591 Human
- Unigene: 503878 Human
- Unigene: 733031 Human
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Alternative names
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)](https://www.abcam.com/ps/products/133/ab133345/Images/ab133345-146900-anti-ncam1-antibody-epr2566-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue staining NCAM with ab133345 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345)](https://www.abcam.com/ps/products/133/ab133345/Images/ab133345-146899-anti-ncam1-antibody-epr2566-western-blot.jpg)
Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345)All lanes : Anti-NCAM1 antibody [EPR2566] (ab133345) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : Human fetal brain cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-rabbit HRP at 1/2000 dilution
Predicted band size: 95 kDa
Observed band size: 140-180 kDa why is the actual band size different from the predicted?
Protocols
References
This product has been referenced in:
- Ongaratti BR et al. Gene and protein expression of E-cadherin and NCAM markers in non-functioning pituitary adenomas. Ann Diagn Pathol 38:59-61 (2019). Read more (PubMed: 30419428) »
- Yuan H et al. HPV positive neuroendocrine cervical cancer cells are dependent on Myc but not E6/E7 viral oncogenes. Sci Rep 7:45617 (2017). IHC-P, IF ; Human . Read more (PubMed: 28378747) »
