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Neuroscience Cell Type Marker Neuron marker Growth Cone
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RecombinantRabMAb

Recombinant Anti-NCAM1 antibody [EPR2566] (ab133345)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)
  • Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345)
  • Anti-NCAM1 antibody [EPR2566] (ab133345)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2566] to NCAM1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-NCAM1 antibody [EPR2566]
    See all NCAM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2566] to NCAM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human NCAM1 aa 250-350 (extracellular). The exact sequence is proprietary.
    Database link: P13591

  • Positive control

    • SH-SY5Y and Human fetal brain cell lysates; Human glioma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR2566
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Growth Cone
    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Neuroscience
    • Neurology process
    • Neural Signal Transduction
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Human Lineage Negative

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-NCAM1 antibody [EPR2566] (ab200333)
    • Alexa Fluor® 594 Anti-NCAM1 antibody [EPR2566] (ab201742)
    • Anti-NCAM1 antibody [EPR2566] - BSA and Azide free (ab214436)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Recombinant Human NCAM1 protein (ab114198)
  • Recombinant Protein

    • Recombinant Human NCAM1 protein (ab114198)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133345 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Detects a band of approximately 140-180 kDa (predicted molecular weight: 95 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Detects a band of approximately 140-180 kDa (predicted molecular weight: 95 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
    • Sequence similarities

      Contains 2 fibronectin type-III domains.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization

      Secreted and Cell membrane.
    • Target information above from: UniProt accession P13591 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4684 Human
      • Omim: 116930 Human
      • SwissProt: P13591 Human
      • Unigene: 503878 Human
      • Alternative names

        • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
        • antigen recognized by monoclonal antibody 5.1H11 antibody
        • CD56 antibody
        • cell adhesion molecule, neural, 1 antibody
        • MSK 39 antibody
        • MSK39 antibody
        • N-CAM-1 antibody
        • NCAM 1 antibody
        • NCAM antibody
        • NCAM C antibody
        • NCAM-1 antibody
        • NCAM1 antibody
        • NCAM1_HUMAN antibody
        • NCAMC antibody
        • Neural cell adhesion molecule 1 antibody
        • Neural cell adhesion molecule NCAM antibody
        • OTTHUMP00000235666 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM1 antibody [EPR2566] (ab133345)

        Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue staining NCAM with ab133345 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345)
        Western blot - Anti-NCAM1 antibody [EPR2566] (ab133345)
        All lanes : Anti-NCAM1 antibody [EPR2566] (ab133345) at 1/1000 dilution

        Lane 1 : SH-SY5Y cell lysate
        Lane 2 : Human fetal brain cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat Anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 95 kDa
        Observed band size: 140-180 kDa why is the actual band size different from the predicted?

      • Anti-NCAM1 antibody [EPR2566] (ab133345)
        Anti-NCAM1 antibody [EPR2566] (ab133345)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (6)

      Publishing research using ab133345? Please let us know so that we can cite the reference in this datasheet.

      ab133345 has been referenced in 6 publications.

      • Li X  et al. Odontogenesis and neuronal differentiation characteristics of periodontal ligament stem cells from beagle dog. J Cell Mol Med 24:5146-5151 (2020). PubMed: 32202359
      • Ongaratti BR  et al. Gene and protein expression of E-cadherin and NCAM markers in non-functioning pituitary adenomas. Ann Diagn Pathol 38:59-61 (2019). PubMed: 30419428
      • Yuan H  et al. HPV positive neuroendocrine cervical cancer cells are dependent on Myc but not E6/E7 viral oncogenes. Sci Rep 7:45617 (2017). IHC-P, IF ; Human . PubMed: 28378747
      • Mendes GA  et al. Expression of E-cadherin, Slug and NCAM and its relationship to tumor invasiveness in patients with acromegaly. Braz J Med Biol Res 51:e6808 (2017). PubMed: 29267504
      • Suarez ER  et al. Chimeric antigen receptor T cells secreting anti-PD-L1 antibodies more effectively regress renal cell carcinoma in a humanized mouse model. Oncotarget 7:34341-55 (2016). PubMed: 27145284
      • Lundgreen K  et al. Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears. Acta Orthop 84:565-70 (2013). IHC-P ; Human . PubMed: 24171689

      Customer reviews and Q&As

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