Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2566] to NCAM1 - BSA and Azide free
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
Product nameAnti-NCAM1 antibody [EPR2566] - BSA and Azide free
See all NCAM1 primary antibodies
DescriptionRabbit monoclonal [EPR2566] to NCAM1 - BSA and Azide free
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
Synthetic peptide within Human NCAM1 aa 250-350 (extracellular). The exact sequence is proprietary.
Database link: P13591
- SH-SY5Y and Human fetal brain cell lysates; Human glioma tissue.
ab214436 is the carrier-free version of ab133345 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab214436 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab214436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 140-180 kDa (predicted molecular weight: 94 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionThis protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
Sequence similaritiesContains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
This IHC data was generated using the same anti-NCAM antibody clone, EPR2566, in a different buffer formulation (cat# ab133345).
Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human glioma tissue staining NCAM with ab133345 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab214436 has been referenced in 1 publication.
- Lundgreen K et al. Lower muscle regenerative potential in full-thickness supraspinatus tears compared to partial-thickness tears. Acta Orthop 84:565-70 (2013). IHC-P ; Human . PubMed: 24171689