Overview

  • Product name
    Anti-NCAM1 antibody [MEM-188] (Allophycocyanin)
    See all NCAM1 primary antibodies
  • Description
    Mouse monoclonal [MEM-188] to NCAM1 (Allophycocyanin)
  • Host species
    Mouse
  • Conjugation
    Allophycocyanin. Ex: 645nm, Em: 660nm
  • Specificity
    ab28335 recognises an isoform of NCAM characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KG-1 human acute myelogenous leukaemia cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab28335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10 µl per 100 µl of whole blood.

 

 

 

ab91364 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.
  • Sequence similarities
    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
    • antigen recognized by monoclonal antibody 5.1H11 antibody
    • CD56 antibody
    • cell adhesion molecule, neural, 1 antibody
    • MSK 39 antibody
    • MSK39 antibody
    • N-CAM-1 antibody
    • NCAM 1 antibody
    • NCAM antibody
    • NCAM C antibody
    • NCAM-1 antibody
    • NCAM1 antibody
    • NCAM1_HUMAN antibody
    • NCAMC antibody
    • Neural cell adhesion molecule 1 antibody
    • Neural cell adhesion molecule NCAM antibody
    • OTTHUMP00000235666 antibody
    see all

References

ab28335 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Flow Cytometry
Sample
Human Cell (PBMC)
Specification
PBMC
Preparation
Cell harvesting/tissue preparation method: Ficoll-Hypaque gradient
Sample buffer: PBS
Permeabilization
No
Gating Strategy
lymphocytes

Abcam user community

Verified customer

Submitted Apr 24 2013

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry these 2 products did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx (ab15160, new lot and ab14047).

To check the status of the order please contact our Customer Service team and reference this number.
Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.
I wish you the best of luck with your research.

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Answer

Thank you very much for contacting us with your question.

We do expectall antibodies from the same clone tohave the same cross-reactivity, so Ab25640 should react with mouse samples despite the difference in conjugation between it and Ab28335. We don't add a species to the datasheet for a particular product untilit is tested, and Ab25640 hasn't yet been tested with mouse samples so only human is listed.

Since Ab25640 hasn't specifically been tested with mouse samples, you are eligible for our testing discount program if you'd like to try it. For this program, you would first purchase ab25640, test it with mouse samples, and submit your feedback to us in an online Abreview. In exchange we will give you one free primary antibody on a future purchase. Please let me know if you're interested in this program and I can set this up for you.

We do not currently hold stock of Ab25640, and we expect it to arrive within 1-2 weeks of placing an order.

I hope that this information is useful, but please let me know if you have further questions or need anything else and I'll be happy to help.

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Answer

Thank you for your call today and for letting us know about this issue.

As we discussed, please keep me updated with the results after using positive and negative control cell lines. I will also try to find any additional information that might explain the discrepancy between these antibodies.

Please let me know if you have any further questions or if there is anything else that we can do for you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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