• Product name
    Anti-NCAM1 antibody [RNL-1]
    See all NCAM1 primary antibodies
  • Description
    Mouse monoclonal [RNL-1] to NCAM1
  • Host species
  • Specificity
    Neural cell adhesion molecule (NCAM). ab9018 reacts with three isoforms of NCAM: NCAM-120, NCAM-140, and NCAM-180.
  • Tested applications
    Suitable for: Flow Cyt, IHC-FoFr, ICC/IF, ICC, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    This antibody was raised against the small lung cancer cell line NCI-H82.

  • Positive control
    • IHC-P: Mouse brain tissue. IHC-FoFr: Rat brain tissue. ICC/IF: Adult-derived human liver stem cells and hepatic stellate cells. Flow cytometry: HT1080 cells.
  • General notes
    Punctuate cytoplasmic staining is observed with ab9018. Some staining of red blood cells observed.



Our Abpromise guarantee covers the use of ab9018 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 2µg for 106 cells.

Paraformaldehyde or methanol fixed cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-FoFr 1/100 - 1/200. see detailed protocol
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
WB 1/100 - 1/1000.
IHC-P Use at an assay dependent concentration.



  • ab9018 staining NCAM in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was not performed. Samples were incubated with primary antibody (1/100) for 12 hours at 4°C. A Biotin-conjugated Rabbit anti-mouse polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • Immunocytochemical analysis of Adult-Derived Human Liver Stem Cells (ADHLSC) in the left image and Hepatic Stellate Cells (HSC) in the right image. ab9018 was used to label NCAM, which is expressed in the HSC. Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. Blocking was with 3.3% hydrogen peroxide for 3 minutes. Permeabilization with D-PBS and 1% Triton X-100 for 10 minutes. Cells incubated with ab9018 at 1/100 for 1 hour. DAB staining. Counterstaining with Mayer's hematoxylin. 

  • Overlay histogram showing HT1080 cells stained with ab9018 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9018, 2µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30µm thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 40x objective and [B] punctate cytoplasmic staining; 20x objective and [C] neuron + processes; x20 objective. No labeling observed following omission of primary antibody. Images coloured in Photoshop.

    NB: We recommend indirect amplification of immunofluorescence for ab9018

    Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).


This product has been referenced in:
  • Eguchi T  et al. Organoids with cancer stem cell-like properties secrete exosomes and HSP90 in a 3D nanoenvironment. PLoS One 13:e0191109 (2018). Read more (PubMed: 29415026) »
  • Wilson RJ  et al. Mitochondrial protein S-nitrosation protects against ischemia reperfusion-induced denervation at neuromuscular junction in skeletal muscle. Free Radic Biol Med 117:180-190 (2018). IHC-Fr ; Mouse . Read more (PubMed: 29432799) »

See all 14 Publications for this product

Customer reviews and Q&As

The antibodies mentioned above are suitable for Rat as well as human samples. ab9018 is yet to be tested in IHC-Fr however as it is tested in IHC-FoFr so it is more likely to work.

The suitable secondary's are for ab4648, ab82259 and ab90...

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I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for a refund for you.

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Thank you for taking the time to contact me again and provide an update. I am sincerely sorry to hear you have also had difficulty obtaining satisfactory results from the replacement vial of antibody.

I appreciate your concerns, this is disa...

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I fully understand your concerns and would like to provide some information that I hope will give some reassurance. I can confirmthat we monitor feedback closely on a weekly basis and we are not currently concerne...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will provide us with vital informa...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forIHC-...

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Thank you for contacting us.

I suggest having a look at some of the references for our NCAM antibodies such as ab9018, to see if this marker will be appropriate for what you are trying to do. It is not clear to me if NCAM is on all neurons o...

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Thank you for contacting us. I am sorry that this antibody did not perform as stated on the datasheet. The above amount will be credited back to your credit card. To avoid confusion, please ensure your accounts department is aware of how the credit...

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Thank you for sending those images. Comparing the images you sent and the images from the Allen Institute for Brain Science, see link below: http://mouse.brain-map.org/gene/show/17734 It would appear that your staining is similar to that theirs, maybe ...

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