Anti-NCAM1 antibody [RNL-1] (ab9018)
Key features and details
- Mouse monoclonal [RNL-1] to NCAM1
- Suitable for: ICC, IHC-FoFr
- Reacts with: Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-NCAM1 antibody [RNL-1]
See all NCAM1 primary antibodies -
Description
Mouse monoclonal [RNL-1] to NCAM1 -
Host species
Mouse -
Specificity
Neural cell adhesion molecule (NCAM). ab9018 reacts with three isoforms of NCAM: NCAM-120, NCAM-140, and NCAM-180. -
Tested applications
Suitable for: ICC, IHC-FoFrmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Tissue, cells or virus corresponding to Human NCAM1. This antibody was raised against the small cell lung cancer cell line NCI-H82.
Database link: P13591 -
Positive control
- IHC-P: Mouse brain tissue. IHC-Fr: Rat brain tissue. ICC/IF: Adult-derived human liver stem cells and hepatic stellate cells.
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General notes
Punctuate cytoplasmic staining is observed with ab9018. Some staining of red blood cells observed.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
RNL-1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab9018 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC | (1) |
1/100 - 1/200.
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IHC-FoFr |
1/100 - 1/200.
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Notes |
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ICC
1/100 - 1/200. |
IHC-FoFr
1/100 - 1/200. |
Target
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Function
This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. -
Sequence similarities
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4684 Human
- Entrez Gene: 24586 Rat
- Omim: 116930 Human
- SwissProt: P13591 Human
- SwissProt: P13596 Rat
- Unigene: 503878 Human
- Unigene: 11283 Rat
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Alternative names
- antigen MSK39 identified by monoclonal antibody 5.1H11 antibody
- antigen recognized by monoclonal antibody 5.1H11 antibody
- CD56 antibody
see all
Images
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Immunohistochemistry of H82 cells, showing staining for NCAM1 using ab9018 (1/500).
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Immunocytochemical analysis of Adult-Derived Human Liver Stem Cells (ADHLSC) in the left image and Hepatic Stellate Cells (HSC) in the right image. ab9018 was used to label NCAM, which is expressed in the HSC. Cells were fixed in 3.5% paraformaldehyde for 15 minutes at room temperature. Blocking was with 3.3% hydrogen peroxide for 3 minutes. Permeabilization with D-PBS and 1% Triton X-100 for 10 minutes. Cells incubated with ab9018 at 1/100 for 1 hour. DAB staining. Counterstaining with Mayer's hematoxylin.
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ab9018 at a dilution of 1/500, staining NCAM (Alexa 488 secondary at 1/2000) on rat brain tissue (30
µ m thick coronal sections) in free floating IHC (see protocol link for detailed description). Images showing neuron body and processes: [A] 40x objective and [B] punctate cytoplasmic staining; 20x objective and [C] neuron + processes; x20 objective. No labeling observed following omission of primary antibody. Images coloured in Photoshop.NB: We recommend indirect amplification of immunofluorescence for ab9018
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab9018 has been referenced in 28 publications.
- Sun JH et al. Nerve bundle formation during the promotion of peripheral nerve regeneration: collagen VI-neural cell adhesion molecule 1 interaction. Neural Regen Res 17:1023-1033 (2022). PubMed: 34558529
- Lee HR et al. CD14+ monocytes and soluble CD14 of synovial fluid are associated with osteoarthritis progression. Arch Rheumatol 37:335-343 (2022). PubMed: 36589618
- Wang J et al. Low immune index correlates with favorable prognosis but with reduced benefit from chemotherapy in gallbladder cancer. Cancer Sci 111:219-228 (2020). PubMed: 31729088
- Rao F et al. Aligned chitosan nanofiber hydrogel grafted with peptides mimicking bioactive brain-derived neurotrophic factor and vascular endothelial growth factor repair long-distance sciatic nerve defects in rats. Theranostics 10:1590-1603 (2020). PubMed: 32042324
- Xie S et al. Hyperion imaging system reveals heterogeneous tumor microenvironment of oral squamous cell carcinoma patients at T1N0M0 stage. Ann Transl Med 8:1513 (2020). PubMed: 33313258