Key features and details
- Rabbit polyclonal to NCAM2
- Suitable for: IHC-P, WB
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Product nameAnti-NCAM2 antibody
See all NCAM2 primary antibodies
DescriptionRabbit polyclonal to NCAM2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human, Pig
Predicted to work with: Mouse
Recombinant fragment corresponding to Human NCAM2 aa 21-297. (N-terminal His-tag and GST-tag; Expressed in E.coli).
LQVTISLSKVELSVGESKFFTCTAIGEPESIDWYNPQGEKIISTQRVVVQ KEGVRSRLTIYNANIEDAGIYRCQATDAKGQTQEATVVLEIYQKLTFREV VSPQEFKQGEDAEVVCRVSSSPAPAVSWLYHNEEVTTISDNRFAMLANNN LQILNINKSDEGIYRCEGRVEARGEIDFRDIIVIVNVPPAISMPQKSFNA TAERGEEMTFSCRASGSPEPAISWFRNGKLIEENEKYILKGSNTELTVRN IINSDGGPYVCRATNKAGEDEKQAFLQ
Database link: O15394
- IHC-P: Human cerebrum and kidney tissues. WB: Recombinant human NCAM2 protein; Pig and rat brain lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232923 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 93 kDa.|
FunctionMay play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.
Tissue specificityExpressed most strongly in adult and fetal brain.
Sequence similaritiesContains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
Cellular localizationCell membrane.
- Information by UniProt
- Cell adhesion molecule, neural, 2 antibody
- MGC51008 antibody
- N CAM 2 antibody
All lanes : Anti-NCAM2 antibody (ab232923) at 1 µg/ml
Lane 1 : Pig brain lysate
Lane 2 : Rat brain lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 93 kDa
Anti-NCAM2 antibody (ab232923) at 1 µg/ml + Recombinant human NCAM2 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 93 kDa
Paraffin-embedded human kidney tissue stained for NCAM2 using ab232923 at 20 µg/ml in immunohistochemical analysis. DAB staining.
Paraffin-embedded human cerebrum tissue stained for NCAM2 using ab232923 at 20 µg/ml in immunohistochemical analysis. DAB staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232923 has not yet been referenced specifically in any publications.