RecombinantRabMAb

Recombinant Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
  • Western blot - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
  • Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
  • Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12815(B)] to NCAM2
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

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Carrier Free

Overview

  • Product name

    Anti-NCAM2 antibody [EPR12815(B)]
    See all NCAM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12815(B)] to NCAM2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7 and Human fetal brain lysates; Human glioma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab173297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 93 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 93 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.

  • Tissue specificity

    Expressed most strongly in adult and fetal brain.

  • Sequence similarities

    Contains 2 fibronectin type-III domains.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cell adhesion molecule, neural, 2 antibody
      • MGC51008 antibody
      • N CAM 2 antibody
      • N CAM 21 antibody
      • N CAM2 antibody
      • N CAM21 antibody
      • N-CAM-2 antibody
      • NCAM 2 antibody
      • NCAM 21 antibody
      • NCAM-2 antibody
      • Ncam2 antibody
      • NCAM2_HUMAN antibody
      • NCAM21 antibody
      • Neural cell adhesion molecule 2 antibody
      • OCAM GPI antibody
      • R4B12 antibody
      • RB 8 neural cell adhesion molecule antibody
      • RB8 neural cell adhesion molecule antibody
      • RNCAM antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

      Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling NCAM2 with ab173297 at 1/50 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
      Western blot - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
      All lanes : Anti-NCAM2 antibody [EPR12815(B)] (ab173297) at 1/1000 dilution

      Lane 1 : MCF7 lysate
      Lane 2 : Human fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 93 kDa

    • Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
      Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

      Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab173297.

    • Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
      Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

    References (0)

    Publishing research using ab173297? Please let us know so that we can cite the reference in this datasheet.

    ab173297 has not yet been referenced specifically in any publications.

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