Recombinant Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NCAM2 antibody [EPR12815(B)]
See all NCAM2 primary antibodies -
Description
Rabbit monoclonal [EPR12815(B)] to NCAM2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NCAM2 aa 800 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: O15394 -
Positive control
- MCF7 and Human fetal brain lysates; Human glioma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12815(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab173297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 93 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. |
Target
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Function
May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons. -
Tissue specificity
Expressed most strongly in adult and fetal brain. -
Sequence similarities
Contains 2 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 4685 Human
- Omim: 602040 Human
- SwissProt: O15394 Human
- Unigene: 473450 Human
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Alternative names
- Cell adhesion molecule, neural, 2 antibody
- MGC51008 antibody
- N CAM 2 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)](https://www.abcam.com/ps/products/173/ab173297/Images/ab173297-196720-anti-ncam2-antibody-epr12815b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling NCAM2 with ab173297 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)](https://www.abcam.com/ps/products/173/ab173297/Images/ab173297-196723-anti-ncam2-antibody-epr12815b-western-blot.jpg)
Western blot - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)All lanes : Anti-NCAM2 antibody [EPR12815(B)] (ab173297) at 1/1000 dilution
Lane 1 : MCF7 lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 93 kDa -
![Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)](https://www.abcam.com/ps/products/173/ab173297/Images/ab173297-196722-anti-ncam2-antibody-epr12815b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab173297.
Protocols
Datasheets and documents
References
ab173297 has not yet been referenced specifically in any publications.
