RecombinantRabMAb

Recombinant Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
  • Western blot - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
  • Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
  • Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12815(B)] to NCAM2
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-NCAM2 antibody [EPR12815(B)]
    See all NCAM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12815(B)] to NCAM2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NCAM2 aa 800 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: O15394

  • Positive control

    • MCF7 and Human fetal brain lysates; Human glioma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab173297 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 93 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 93 kDa.
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.

    • Target

    • Function

      May play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons.

    • Tissue specificity

      Expressed most strongly in adult and fetal brain.

    • Sequence similarities

      Contains 2 fibronectin type-III domains.
      Contains 5 Ig-like C2-type (immunoglobulin-like) domains.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cell adhesion molecule, neural, 2 antibody
        • MGC51008 antibody
        • N CAM 2 antibody
        • N CAM 21 antibody
        • N CAM2 antibody
        • N CAM21 antibody
        • N-CAM-2 antibody
        • NCAM 2 antibody
        • NCAM 21 antibody
        • NCAM-2 antibody
        • Ncam2 antibody
        • NCAM2_HUMAN antibody
        • NCAM21 antibody
        • Neural cell adhesion molecule 2 antibody
        • OCAM GPI antibody
        • R4B12 antibody
        • RB 8 neural cell adhesion molecule antibody
        • RB8 neural cell adhesion molecule antibody
        • RNCAM antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

        Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling NCAM2 with ab173297 at 1/50 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
        Western blot - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
        All lanes : Anti-NCAM2 antibody [EPR12815(B)] (ab173297) at 1/1000 dilution

        Lane 1 : MCF7 lysate
        Lane 2 : Human fetal brain lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 93 kDa

      • Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
        Immunoprecipitation - Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

        Western blot analysis on immunoprecipitation pellet from fetal brain lysate using ab173297.

      • Anti-NCAM2 antibody [EPR12815(B)] (ab173297)
        Anti-NCAM2 antibody [EPR12815(B)] (ab173297)

      References (0)

      Publishing research using ab173297? Please let us know so that we can cite the reference in this datasheet.

      ab173297 has not yet been referenced specifically in any publications.

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