Key features and details
- Rabbit polyclonal to NCAPD2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NCAPD2 antibody
See all NCAPD2 primary antibodies
DescriptionRabbit polyclonal to NCAPD2
Tested applicationsSuitable for: WBmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Predicted to work with: Mouse
- This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat.
This product was previously labelled as CNAP1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab34307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This antibody does not appear to be suitable for ICC/IF (please see abreview).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceCNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
Cellular localizationNuclear and cytoplasmic.
- 2810406C15Rik antibody
- 2810465G24Rik antibody
- CAP-D2 antibody
All lanes : Anti-NCAPD2 antibody (ab34307) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 :
Jurkat whole cell lysate (ab7899) at 20 µg
All lanes : IR Dye 680 conjugated Goat-Anti Rabbit IgG (H&L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 157 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
ab34307 has not yet been referenced specifically in any publications.