Overview

  • Product name

  • Description

    Rabbit polyclonal to NCAPD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human NCAPD2 aa 1200-1300 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab34336, ab34337)

  • Positive control

    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat.
  • General notes

     This product was previously labelled as CNAP1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab34307 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa).

    This antibody does not appear to be suitable for ICC/IF (please see abreview).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance

      CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
    • Cellular localization

      Nuclear and cytoplasmic.
    • Database links

    • Alternative names

      • 2810406C15Rik antibody
      • 2810465G24Rik antibody
      • CAP-D2 antibody
      • CAPD2 antibody
      • CEP2 antibody
      • CEP250 antibody
      • Chromosome condensation-related SMC-associated protein 1 antibody
      • Chromosome-associated protein D2 antibody
      • CNAP1 antibody
      • CND1 antibody
      • Condensin antibody
      • Condensin complex subunit 1 antibody
      • eg7 antibody
      • hCAP-D2 antibody
      • hCAPD2 antibody
      • KIAA0159 antibody
      • mKIAA0159 antibody
      • NCAPD2 antibody
      • non-SMC condensin I complex, subunit D2 antibody
      • XCAP D2 homolog antibody
      see all

    Images

    • All lanes : Anti-NCAPD2 antibody (ab34307) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
      Lane 2 : Jurkat whole cell lysate (ab7899) at 20 µg

      Secondary
      All lanes : IR Dye 680 conjugated Goat-Anti Rabbit IgG (H&L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 157 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?

    References

    ab34307 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Mar 05 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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