Overview

  • Product name

  • Description

    Rabbit polyclonal to NCAPD2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human NCAPD2 aa 1350 to the C-terminus (C terminal).
    (Peptide available as ab34337)

  • Positive control

    • HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.
  • General notes

     This product was previously labelled as CNAP1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab34338 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa).
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Relevance

      CNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
    • Cellular localization

      Nuclear and cytoplasmic.
    • Database links

    • Alternative names

      • 2810406C15Rik antibody
      • 2810465G24Rik antibody
      • CAP-D2 antibody
      • CAPD2 antibody
      • CEP2 antibody
      • CEP250 antibody
      • Chromosome condensation-related SMC-associated protein 1 antibody
      • Chromosome-associated protein D2 antibody
      • CNAP1 antibody
      • CND1 antibody
      • Condensin antibody
      • Condensin complex subunit 1 antibody
      • eg7 antibody
      • hCAP-D2 antibody
      • hCAPD2 antibody
      • KIAA0159 antibody
      • mKIAA0159 antibody
      • NCAPD2 antibody
      • non-SMC condensin I complex, subunit D2 antibody
      • XCAP D2 homolog antibody
      see all

    Images

    • Lane 1 : Marker
      Lanes 2-4 : Anti-NCAPD2 antibody (ab34338) at 1 µg/ml

      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
      Lane 3 : Jurkat whole cell lysate (ab7899) at 20 µg
      Lane 4 : A431 whole cell lysate (ab7909) at 20 µg

      Secondary
      Lanes 2-4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution

      Performed under reducing conditions.

      Predicted band size: 157 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?

    • Image courtesy of Human Protein Atlas ab34338 staining NCAPD2 in human testis, showing a predominantly nuclear staining pattern in cells of the seminiferous tubule. Paraffin embedded human testis tissue was incubated with ab34338 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

      ab34338 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org

    References

    ab34338 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Question
    Answer

    Thank you for contacting us.
    As discussed please let me know if you are interested in the 100% Abreview testing discount programme.
    Please click the link for more details;
    https://www.abcam.com/index.html?pageconfig=resource&rid=11998&viapagetrap=collaborationdiscount
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
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    Read More

    Answer

    Thank you for contacting us. Your credit note ID is XXXXXX for ab34338 and XXXXXX for ab4448.  I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of the following ways: (1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding. Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge. To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website. The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice  

    Read More

    Answer

    Thank you for confirming the details. The antibodies should be working fine with the protocol details you have provided. I am not sure why these antibodies fail. I am happy in sending replacement vials of both of these antibodies. Could you let me know how you would like to proceed? I would also like to draw your attention for storing these antibodies please store at -20C after liquating these and in case you need to keep them 4C please try to use aliquots within 1-2 weeks. I hope these suggestions will be helpful.

    Read More

    Answer

    Thank you for your enquiry regarding ab4448 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Ab4448 is a very high characterized antibody which has been used in tens of publications and Abreview; failing of this antibody actually surprises us. Even ab34338 was tested successfully with human lysates so this antibody should also be fine with human cells.   We unfortunately can’t guarantee the use of these antibodies for Dog species. I have read the protocol which looks fine to me however I would like to ask few questions which would help me identify the source of the problem; - Could you provide details e.g. at what temperature the antibodies were stored? - We have used Hela cells to test these antibodies; this is a easily available cell line which I would recommend using as a positive control. - You have mentioned using Tubulin as positive control. How the tubulin staining was? - Does these antibodies fail to give staining at all or giving high background? - For how long the cells were permeabilized. - Have you tried same procedure successfully before? - Why the reporting of problem was delayed for 8 months. I will look forward to hearing from you soon.

    Read More
    This product is known to not work in this application or species.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Mar 05 2007

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