Product nameAnti-NCAPD2 antibody
See all NCAPD2 primary antibodies
DescriptionRabbit polyclonal to NCAPD2
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide corresponding to Human NCAPD2 aa 1350 to the C-terminus (C terminal).
(Peptide available as
- HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate, Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate, A431 (Human epithelial carcinoma cell line) Whole Cell Lysate.
This product was previously labelled as CNAP1
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab34338 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa).|
RelevanceCNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
Cellular localizationNuclear and cytoplasmic.
- 2810406C15Rik antibody
- 2810465G24Rik antibody
- CAP-D2 antibody
Lane 1 : Marker
Lanes 2-4 : Anti-NCAPD2 antibody (ab34338) at 1 µg/ml
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 3 :
Jurkat whole cell lysate (ab7899) at 20 µg
Lane 4 :
A431 whole cell lysate (ab7909) at 20 µg
Lanes 2-4 : IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 157 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Image courtesy of Human Protein Atlas ab34338 staining NCAPD2 in human testis, showing a predominantly nuclear staining pattern in cells of the seminiferous tubule. Paraffin embedded human testis tissue was incubated with ab34338 (1/100 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab34338 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues. Further results for this antibody can be found at www.proteinatlas.org
ab34338 has not yet been referenced specifically in any publications.