Product nameAnti-NCAPD2 antibody [EPR9220]
See all NCAPD2 primary antibodies
DescriptionRabbit monoclonal [EPR9220] to NCAPD2
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human NCAPD2 aa 100-200. The exact sequence is proprietary.
- HeLa and Jurkat cell lysates.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as CNAP1
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 157 kDa.|
RelevanceCNAP1/hCAP-D2 is a regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condensed chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. It may target the condensin complex to DNA via its C-terminal domain (referenced from Swissprot).
Cellular localizationNuclear and cytoplasmic.
- 2810406C15Rik antibody
- 2810465G24Rik antibody
- CAP-D2 antibody
All lanes : Anti-NCAPD2 antibody [EPR9220] (ab137075) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 157 kDa
Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling NCAPD2 with ab137075 at a dilution of 1/400. Cells were fixed with methanol. ab150081 (1/200) was used as the secondary antibody.
This antibody produces a strong, chromatin-associated labelling pattern in mitotic (prophase to metaphase) MeOH fixed samples. It does not produce the expected labelling pattern in PF fixed samples.
ab137075 has not yet been referenced specifically in any publications.