Overview

  • Product name

  • Description

    Rabbit polyclonal to NCAPG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NCAPG (internal sequence). The exact sequence is proprietary.
    Database link: Q9BPX3

  • Positive control

    • WB: HEK-293T and A431 whole cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab226805 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 114 kDa.

Target

  • Function

    Regulatory subunit of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases.
  • Tissue specificity

    Highly expressed in testis.
  • Sequence similarities

    Belongs to the CND3 (condensin subunit 3) family.
    Contains 10 HEAT repeats.
  • Post-translational
    modifications

    Phosphorylated by CDK1. Its phosphorylation, as well as that of NCAPD2 and NCAPH subunits, activates the condensin complex and is required for chromosome condensation.
  • Cellular localization

    Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDK1, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAPG antibody
    • CHCG antibody
    • Chromosome associated protein G antibody
    • Chromosome condensation protein G antibody
    • Chromosome-associated protein G antibody
    • CND3_HUMAN antibody
    • Condensin complex subunit 3 antibody
    • Condensin subunit CAP G antibody
    • Condensin subunit CAP-G antibody
    • HCAP G antibody
    • hCAP-G antibody
    • Melanoma antigen NY MEL 3 antibody
    • Melanoma antigen NY-MEL-3 antibody
    • NCAPG antibody
    • Non SMC condensin I complex subunit G antibody
    • Non-SMC condensin I complex subunit G antibody
    • NY MEL 3 antibody
    • XCAP G homolog antibody
    • XCAP-G homolog antibody
    see all

Images

  • All lanes : Anti-NCAPG antibody (ab226805) at 1/2000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 114 kDa



    5% SDS-PAGE gel.

References

ab226805 has not yet been referenced specifically in any publications.

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