Anti-NCAPH2 antibody (ab221722)
Key features and details
- Rabbit polyclonal to NCAPH2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NCAPH2 antibody
See all NCAPH2 primary antibodies -
Description
Rabbit polyclonal to NCAPH2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NCAPH2 aa 350-450.
Database link: Q6IBW4 -
Positive control
- WB: Human liver lysate. ICC/IF: U-2 OS cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 68 kDa.
|
|
ICC/IF | (1) |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
---|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 68 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
-
Function
Regulatory subunit of the condensin-2 complex, a complex that seems to provide chromosomes with an additional level of organization and rigidity and in establishing mitotic chromosome architecture. May play a role in lineage-specific role in T-cell development. -
Sequence similarities
Belongs to the CND2 H2 (condensin-2 subunit 2) family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. Chromosome. Distributed along the arms of chromosomes assembled in vivo and in vitro. - Information by UniProt
-
Database links
- Entrez Gene: 29781 Human
- Omim: 611230 Human
- SwissProt: Q6IBW4 Human
- Unigene: 180903 Human
-
Alternative names
- CAP H2 antibody
- CAP H2 subunit of the condensin II complex antibody
- CAPH2 antibody
see all
Images
-
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for NCAPH2 (green) using ab221722 at 4 µg/ml in ICC/IF.
-
All lanes : Anti-NCAPH2 antibody (ab221722) at 1/500 dilution
Lane 1 : RT4 (human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (human brain glioma cell line) cell lysate
Lane 3 : Human plasma
Lane 4 : Human liver lysate
Predicted band size: 68 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab221722 has not yet been referenced specifically in any publications.