Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein G purified
Purification notesab84854 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 µm membrane.
Our Abpromise guarantee covers the use of ab84854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Dot blot||Use at an assay dependent dilution.|
|ICC/IF||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.|
RelevanceNCBP2 is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. It has an RNP domain commonly found in RNA binding proteins, and contains the cap-binding activity.
Cellular localizationCytoplasmic and Nuclear
- 20 kDa nuclear cap binding protein antibody
- CBC2 antibody
- CBP20 antibody
Anti-NCBP2 antibody [2613C3a] (ab84854) + Immunogen (Recombinant Human NCBP2 fragment)
Predicted band size: 18 kDa
Observed band size: 35 kDa why is the actual band size different from the predicted?
ab84854 staining NCBP2 in HeLa cells by Immunofluorescence. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS.
ab84854 has not yet been referenced specifically in any publications.