Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab15707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAccepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Tissue specificityWidely expressed (at protein level).
PathwayProtein modification; protein neddylation.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
- Information by UniProt
- NCE2 antibody
- NEDD8 carrier protein UBE2F antibody
- NEDD8 conjugating enzyme antibody
All lanes : Anti-NCE2 antibody (ab15707) at 1/1000 dilution
Lane 1 : Endogenous Nce2 from mouse 3T3 cells
Lane 2 : NIH 3T3 cells infected with ShRNA of mouse Nce2
Lane 3 : NIH3T3 cells infected with ShRNA of Nce2 and MCSV-NCE2his flag-IRES-GFP (human NCE2)
Lysates/proteins at 20 µg per lane.
All lanes : HRP-conjugated rabbit polyclonal to goat IgG
Developed using the ECL technique.
Exposure time: 10 minutes
The image is a courtsey of an abreview submitted by Dr. Martine Roussel.
This product has been referenced in:
- Stanley DJ et al. Inhibition of a NEDD8 Cascade Restores Restriction of HIV by APOBEC3G. PLoS Pathog 8:e1003085 (2012). WB . Read more (PubMed: 23300442) »
- Huang DT et al. E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification. Mol Cell 33:483-95 (2009). WB . Read more (PubMed: 19250909) »