Overview

  • Product name
  • Description
    Goat polyclonal to NCE2
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Dog
  • Immunogen

    Peptide with sequence C-

    HLRDKEDFRNKVDD

    , corresponding to C terminal amino acids 165-178 of human NCE2 (NP_542409.1).

  • Positive control
    • Mouse liver lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab87640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 21 kDa).
ELISA 1/32000. Peptide ELISA
IHC-P Use a concentration of 2.5 - 3.8 µg/ml.

Target

  • Function
    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
  • Tissue specificity
    Widely expressed (at protein level).
  • Pathway
    Protein modification; protein neddylation.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • NCE2 antibody
    • NEDD8 carrier protein UBE2F antibody
    • NEDD8 conjugating enzyme antibody
    • NEDD8 conjugating enzyme UBE2F antibody
    • NEDD8 protein ligase UBE2F antibody
    • NEDD8-conjugating enzyme 2 antibody
    • NEDD8-conjugating enzyme UBE2F antibody
    • ube2f antibody
    • UBE2F_HUMAN antibody
    • Ubiquitin conjugating enzyme E2F antibody
    • Ubiquitin conjugating enzyme E2F (putative) antibody
    • Ubiquitin-conjugating enzyme E2 F antibody
    see all

Images

  • Anti-NCE2 antibody (ab87640) at 0.05 µg/ml + mouse liver lysate (in RIPA buffer) at 35 µg

    Developed using the ECL technique.

    Predicted band size: 21 kDa
    Observed band size: 20 kDa (why is the actual band size different from the predicted?)


    Exposure time: 1 hour
  • In paraffin embedded Human Placenta shows membrane staining in trophoblasts-. Recommended concentration, 2.5-3.8µg/ml.

References

ab87640 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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