Overview

  • Product name

    Anti-NCE2/UBE2F antibody - C-terminal
    See all NCE2/UBE2F primary antibodies
  • Description

    Rabbit polyclonal to NCE2/UBE2F - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide within Human NCE2/UBE2F (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
    Database link: Q969M7

  • Positive control

    • WB: NCE2/UBE2F-transfected HEK-293T lysate.
  • General notes

     This product was previously labelled as NCE2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab228626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000.

Target

  • Function

    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
  • Tissue specificity

    Widely expressed (at protein level).
  • Pathway

    Protein modification; protein neddylation.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
  • Information by UniProt
  • Database links

  • Alternative names

    • NCE2 antibody
    • NEDD8 carrier protein UBE2F antibody
    • NEDD8 conjugating enzyme antibody
    • NEDD8 conjugating enzyme UBE2F antibody
    • NEDD8 protein ligase UBE2F antibody
    • NEDD8-conjugating enzyme 2 antibody
    • NEDD8-conjugating enzyme UBE2F antibody
    • ube2f antibody
    • UBE2F_HUMAN antibody
    • Ubiquitin conjugating enzyme E2F antibody
    • Ubiquitin conjugating enzyme E2F (putative) antibody
    • Ubiquitin-conjugating enzyme E2 F antibody
    see all

Images

  • All lanes : Anti-NCE2/UBE2F antibody - C-terminal (ab228626) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
    Lane 2 : NCE2/UBE2F-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate


    12% SDS-PAGE gel.

References

ab228626 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228626.
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