Key features and details
- Rabbit polyclonal to NCE2/UBE2F - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NCE2/UBE2F antibody - C-terminal
See all NCE2/UBE2F primary antibodies
DescriptionRabbit polyclonal to NCE2/UBE2F - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rhesus monkey
Synthetic peptide within Human NCE2/UBE2F (C terminal). The exact sequence is proprietary. Carrier-protein conjugated.
Database link: Q969M7
- WB: NCE2/UBE2F-transfected HEK-293T lysate.
This product was previously labelled as NCE2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228626 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000.|
FunctionAccepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Tissue specificityWidely expressed (at protein level).
PathwayProtein modification; protein neddylation.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
- Information by UniProt
- NCE2 antibody
- NEDD8 carrier protein UBE2F antibody
- NEDD8 conjugating enzyme antibody
All lanes : Anti-NCE2/UBE2F antibody - C-terminal (ab228626) at 1/500 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2 : NCE2/UBE2F-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
12% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228626 has not yet been referenced specifically in any publications.