Recombinant
RabMAb

Recombinant Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)

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Overview

  • Product name

    Anti-NCE2/UBE2F antibody [EPR12932]
    See all NCE2/UBE2F primary antibodies

  • Description

    Rabbit monoclonal [EPR12932] to NCE2/UBE2F

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human NCE2/UBE2F aa 1-150 (N terminal). The exact sequence is proprietary.
    Database link: Q969M7

  • Positive control

    • Jurkat, A375 and NTERA2/D1 cell lysates; Human brain and Human renal carcinoma tissues.

  • General notes

     

     

     This product was previously labelled as NCE2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab185234 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt 1/160.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/250.

Target

  • Function

    Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

  • Tissue specificity

    Widely expressed (at protein level).

  • Pathway

    Protein modification; protein neddylation.

  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family. UBE2F subfamily.
  • Information by UniProt

  • Database links

    • Alternative names

      • NCE2 antibody
      • NEDD8 carrier protein UBE2F antibody
      • NEDD8 conjugating enzyme antibody
      • NEDD8 conjugating enzyme UBE2F antibody
      • NEDD8 protein ligase UBE2F antibody
      • NEDD8-conjugating enzyme 2 antibody
      • NEDD8-conjugating enzyme UBE2F antibody
      • ube2f antibody
      • UBE2F_HUMAN antibody
      • Ubiquitin conjugating enzyme E2F antibody
      • Ubiquitin conjugating enzyme E2F (putative) antibody
      • Ubiquitin-conjugating enzyme E2 F antibody
      see all

    Images

    • Western blot - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)
      Western blot - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)
      All lanes : Anti-NCE2/UBE2F antibody [EPR12932] (ab185234) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : NTERA2/D1 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 21 kDa

    • Flow Cytometry - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)
      Flow Cytometry - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)

      Flow cytometric analysis of A375 cells (2% paraformaldehyde-fixed) labeling NCE2/UBE2F with ab185234 at 1/160 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)
      Immunocytochemistry/ Immunofluorescence - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)

      Immunofluorescent analysis of A375 cells (4% paraformaldehyde-fixed) labeling NCE2/UBE2F with ab185234 at 1/250 dilution followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)

      Immunohistochemical analysis of paraffin-embedded Human renal carcinoma labeling NCE2/UBE2F with ab185234 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCE2/UBE2F antibody [EPR12932] (ab185234)

      Immunohistochemical analysis of paraffin-embedded Human brain labeling NCE2/UBE2F with ab185234 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    References

    This product has been referenced in:

    • Zhou W  et al. UBE2M Is a Stress-Inducible Dual E2 for Neddylation and Ubiquitylation that Promotes Targeted Degradation of UBE2F. Mol Cell 70:1008-1024.e6 (2018). Read more (PubMed: 29932898) »
    See 1 Publication for this product

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