Product nameAnti-NCF1 / p47-phox antibody
See all NCF1 / p47-phox primary antibodies
DescriptionRabbit polyclonal to NCF1 / p47-phox
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human NCF1/ p47-phox.
MGDTFIRHIALLGFEKRFVPSQHYVYMFLVKWQDLSEKVVYRRFTEIYEF HKTLKEMFPIEAGAINPENRIIPHLPAPKWFDGQRAAENRQGTLTEYCGT LMSLPTKISRCPHLLDFFKVRPDDLKLPTDNQTKKPETYLMPKDGKSTAT DITGPIILQTYRAIANYEKTSGSEMALSTG DVVEVVEKSESGWWFCQM KAKRGWIPASFLEPLDSPDETEDPEPNYAGEPYVAIKAYTAVEGDEVSLL EGEAVEVIHKLLDGWWVIRKDDVTGYFPSMYLQKSGQDVSQAQRQIKRGA PPRRSSIRNAHSIHQRSRKRLSQDAYRRNSVRFLQQRRRQARPGPQSPGS PLEEERQTQRSKPQPAVPPRPSADLILNRCSESTKRKLASAV
Database link: P14598
- Recombinant protein; RAW 264.7 cell lysate.
Previously labelled as NCF1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab176483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).|
FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Involvement in diseaseDefects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Sequence similaritiesContains 1 PX (phox homology) domain.
Contains 2 SH3 domains.
- Information by UniProt
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
All lanes : Anti-Transglutaminase 2 antibody (ab176463) at 1/1000 dilution
Lane 1 : Recombinant His-tagged NCF1 protein
Lane 2 : RAW 264.7 cell lysate
Developed using the ECL technique.
Predicted band size: 45 kDa
ab176483 has not yet been referenced specifically in any publications.