Key features and details
- Goat polyclonal to NCF1/p47-phox
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NCF1/p47-phox antibody
See all NCF1/p47-phox primary antibodies
DescriptionGoat polyclonal to NCF1/p47-phox
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Guinea pig, Cow, Dog, Pig, Chinese hamster
- WB: Daudi cell lydates ICC/IF: RAW264.7 and NIH3T3 cells
Previously labelled as NCF1.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab166930 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab166930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 0.03 - 0.1 µg/ml. Predicted molecular weight: 45 kDa.
1 hour primary incubation is recommended for this product.
FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Involvement in diseaseDefects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Sequence similaritiesContains 1 PX (phox homology) domain.
Contains 2 SH3 domains.
- Information by UniProt
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
Anti-NCF1/p47-phox antibody (ab166930) at 1 µg/ml + Daudi cell lysate (in RIPA buffer) at 35 µg
Predicted band size: 45 kDa
Immunocytochemistry/ Immunofluorescence analysis of NIH3T3 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL).
Immunocytochemistry/ Immunofluorescence analysis of RAW264.7 cells labeling NCF1/p47-phox ab166930 at 10ug/mL (1hr). Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. An Alexa Fluor 488 conjugated antibody at 2ug/mL was used as the secondary antibody. The images is showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/mL).
ab166930 has not yet been referenced specifically in any publications.