Anti-NCF1/p47-phox antibody (ab795)
Key features and details
- Goat polyclonal to NCF1/p47-phox
- Suitable for: WB
- Reacts with: Mouse, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-NCF1/p47-phox antibody
See all NCF1/p47-phox primary antibodies -
Description
Goat polyclonal to NCF1/p47-phox -
Host species
Goat -
Tested Applications & Species
Application Species Flow Cyt PigWB MouseHuman -
Immunogen
Synthetic peptide corresponding to Human NCF1/p47-phox aa 378-390 (C terminal).
Sequence:CSESTKRKLASAV
(Peptide available asab22781) -
Positive control
- WB: U937, Daudi, and U251 cell lysates, Mouse thymus and pig spleen tissue lysates.
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General notes
Principal Names - NCF1 / p47-phox; NCF1; p47phox; Neutrophil cytosolic factor-1 (47 kDa, chronic granulomatous disease, autosomal 1) Official Gene Symbol - NCF1 GenBank Accession Number – NP_000256 LocusLink ID - 4687
NCF1 / p47-phox is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils.
Previously labelled as NCF1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Primary antibody notes
NCF1 is a cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
Flow Cyt |
Pig
|
WB |
Mouse
Human
|
All applications |
Rat
Rabbit
Cow
|
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
Involvement in disease
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
Sequence similarities
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 653361 Human
- Entrez Gene: 17969 Mouse
- Entrez Gene: 114553 Rat
- Omim: 608512 Human
- SwissProt: P14598 Human
- SwissProt: Q09014 Mouse
- SwissProt: F1M707 Rat
- Unigene: 647047 Human
see all -
Alternative names
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
see all
Images
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Lane 1 : Anti-NCF1/p47-phox antibody (ab795) at 0.2 µg/ml
Lane 2 : Anti-NCF1/p47-phox antibody (ab795) at 0.01 µg/ml
Lanes 3-4 : Anti-NCF1/p47-phox antibody (ab795) at 0.3 µg/ml
Lane 1 : U937 (human histiocytic lymphoma cell line) cell lysate
Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate
Lane 3 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDaLysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
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All lanes : Anti-NCF1/p47-phox antibody (ab795) at 1 µg/ml
Lane 1 : Mouse thymus lysate
Lane 2 : Pig spleen lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 45 kDaLysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.
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Anti-NCF1/p47-phox antibody (ab795) at 1.5 µg/ml +
Recombinant Human NCF1/p47-phox protein (ab98054) at 0.1 µg
Secondary
Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 45 kDa
Exposure time: 8 minutes
Datasheets and documents
References (11)
ab795 has been referenced in 11 publications.
- Lin H et al. Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition. Aging (Albany NY) 12:12684-12702 (2020). PubMed: 32629426
- Wu W et al. Xian-Ling-Gu-Bao induced inflammatory stress rat liver injury: Inflammatory and oxidative stress playing important roles. J Ethnopharmacol 239:111910 (2019). PubMed: 31026554
- Srivastava S et al. Mycobacterium tuberculosis PPE2 Protein Interacts with p67phox and Inhibits Reactive Oxygen Species Production. J Immunol 203:1218-1229 (2019). PubMed: 31375544
- Ren Y et al. Si-Miao-Yong-An decoction ameliorates cardiac function through restoring the equilibrium of SOD and NOX2 in heart failure mice. Pharmacol Res 146:104318 (2019). PubMed: 31228552
- Kong M et al. Serum response factor (SRF) promotes ROS generation and hepatic stellate cell activation by epigenetically stimulating NCF1/2 transcription. Redox Biol 26:101302 (2019). PubMed: 31442911