Anti-NCF1/p47-phox antibody (ab795)

Reviews (2)Q&A (4)References (15)
Western blot - Anti-NCF1/p47-phox antibody (ab795)
  • Western blot - Anti-NCF1/p47-phox antibody (ab795)
  • Western blot - Anti-NCF1/p47-phox antibody (ab795)

Key features and details

  • Goat polyclonal to NCF1/p47-phox
  • Suitable for: WB
  • Reacts with: Mouse, Human, Pig
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-NCF1/p47-phox antibody
    See all NCF1/p47-phox primary antibodies

  • Description

    Goat polyclonal to NCF1/p47-phox

  • Host species

    Goat

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Human, Pig
    Predicted to work with: Rat, Rabbit, Cow

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: U937, Daudi, and U251 cell lysates, Mouse thymus and pig spleen tissue lysates.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

1 hour primary incubation is recommended for this product.
Notes
WB
Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

1 hour primary incubation is recommended for this product.

Target

  • Function

    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

  • Involvement in disease

    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

  • Sequence similarities

    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

Images

  • Western blot - Anti-NCF1/p47-phox antibody (ab795)
    Western blot - Anti-NCF1/p47-phox antibody (ab795)
    Lane 1 : Anti-NCF1/p47-phox antibody (ab795) at 0.2 µg/ml
    Lane 2 : Anti-NCF1/p47-phox antibody (ab795) at 0.01 µg/ml
    Lanes 3-4 : Anti-NCF1/p47-phox antibody (ab795) at 0.3 µg/ml

    Lane 1 : U937 (human histiocytic lymphoma cell line) cell lysate
    Lane 2 : Daudi (human Burkitt's lymphoma cell line) cell lysate
    Lane 3 : U-251 MG (formally U-373 MG) (human brain glioma cell line) whole cell lysate
    Lane 4 : A431 (human epidermoid carcinoma cell line) whole cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 45 kDa



    Lysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

  • Western blot - Anti-NCF1/p47-phox antibody (ab795)
    Western blot - Anti-NCF1/p47-phox antibody (ab795)
    All lanes : Anti-NCF1/p47-phox antibody (ab795) at 1 µg/ml

    Lane 1 : Mouse thymus lysate
    Lane 2 : Pig spleen lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 45 kDa



    Lysates prepared in RIPA buffer. Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

  • Western blot - Anti-NCF1/p47-phox antibody (ab795)
    Western blot - Anti-NCF1/p47-phox antibody (ab795)
    Anti-NCF1/p47-phox antibody (ab795) at 1.5 µg/ml + Recombinant Human NCF1/p47-phox protein (ab98054) at 0.1 µg

    Secondary
    Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa


    Exposure time: 8 minutes

References (15)

Publishing research using ab795? Please let us know so that we can cite the reference in this datasheet.

ab795 has been referenced in 15 publications.

  • Liu Y  et al. Proteomic Analysis Revealed the Characteristics of Key Proteins Involved in the Regulation of Inflammatory Response, Leukocyte Transendothelial Migration, Phagocytosis, and Immune Process during Early Lung Blast Injury. Oxid Med Cell Longev 2021:8899274 (2021). PubMed: 34007409
  • Lin H  et al. Silencing of long non-coding RNA Sox2ot inhibits oxidative stress and inflammation of vascular smooth muscle cells in abdominal aortic aneurysm via microRNA-145-mediated Egr1 inhibition. Aging (Albany NY) 12:12684-12702 (2020). PubMed: 32629426
  • Song P  et al. Geniposide protects against sepsis-induced myocardial dysfunction through AMPKa-dependent pathway. Free Radic Biol Med 152:186-196 (2020). PubMed: 32081748
  • Cammisotto V  et al. PCSK9 Regulates Nox2-Mediated Platelet Activation via CD36 Receptor in Patients with Atrial Fibrillation. Antioxidants (Basel) 9:N/A (2020). PubMed: 32252393
  • Chen GP  et al. Geranylgeranyl Transferase-I Knockout Inhibits Oxidative Injury of Vascular Smooth Muscle Cells and Attenuates Diabetes-Accelerated Atherosclerosis. J Diabetes Res 2020:7574245 (2020). PubMed: 32851097
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Western blot abreview for Anti-NCF1/p47-phox antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Abcam user community

Verified customer

Submitted Feb 01 2016

Western blot abreview for Anti-NCF1/p47-phox antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 01 2016

Question

Are there any antibodies available that detect the phosphorylated form of p47phox (a regulatory NADPH oxidase subunit) in mouse tissue? I have attached a recent paper which has published in antioxidants and redox signaling. Authors used an antibody from your company for the detection of phospho p47phox in cells extracted from humans.



Thanks,

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Answer

Thank you for contacting us.

Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

The antibodies we have are; ab74095, ab63331, ab111855, ab115564, ab795, ab63554, ab107125. Please check the datasheets and let me know, if you want me to create a performa invoice for you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Sehr geehrte Damen und Herren, wir möchten in unserer Arbeitsgruppe beginnen, mit der NADPH-oxidase in Makrophagen (Rattenmakrophagen NR8383, ev. auch humane Monozyten/Makrophagen U937) zu arbeiten und sind auf der Suche nach Antikörpern gegen ihre Bestandteile (p47PHOX, p40PHOX, p67PHOX, P22PHOX, gp91PHOX, Rac1/2, Rho-GDI, …), sowie (falls möglich) die Einzelkomponenten selber (für die Arbeit im zellfreien System). Können Sie uns weiterhelfen? Über ein Angebot würden wir uns freuen.

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Answer

Vielen Dank für Ihre Anfrage. Gegen die genannten NADPH-Oxidase-Komponenten haben wir folgende Antikörper bzw. Proteine in unserem Katalog: Click here (or use the following: https://www.abcam.com/NOXA2p67phox-antibody-EPR5064-ab109366.html). Click here (or use the following: https://www.abcam.com/NOX2gp91phox-antibody-ab80508.html). Click here (or use the following: https://www.abcam.com/Rac1-antibody-23A8-ab33186.html). Click here (or use the following: https://www.abcam.com/RAC2-antibody-ab2244.html). Click here (or use the following: https://www.abcam.com/RhoGDI-antibody-OTI1F2-ab118159.html). Click here (or use the following: https://www.abcam.com/Cytochrome-b245-Light-Chainp22-phox-antibody-ab75941.html). Click here (or use the following: https://www.abcam.com/p40-phox-antibody-ab115855.html). Click here (or use the following: https://www.abcam.com/p40-phox-phospho-T154-antibody-ab59355.html). Click here (or use the following: https://www.abcam.com/NCF1p47-phox-phospho-S328-antibody-ab111855.html). Click here (or use the following: https://www.abcam.com/NCF1p47-phox-antibody-ab795.html). Click here (or use the following: https://www.abcam.com/Human-NCF4-full-length-protein-ab88031.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NCF1p47-phox-protein-ab98054.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NOXA2p67phox-protein-ab117065.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-NOX2gp91phox-protein-ab114183.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab84657.html). Click here (or use the following: https://www.abcam.com/Human-Rac1-full-length-protein-ab40471.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab89246.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab82670.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-Rac1-protein-ab51014.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab84655.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab78684.html). Click here (or use the following: https://www.abcam.com/Recombinant-Human-RAC2-protein-ab87665.html). Vielleicht interessiert Sie in diesem Zusammenhang auch einer unserer Assaykits (NADP/NADPH): Click here (or use the following: https://www.abcam.com/NADPNADPH-Assay-Kit-ab65349.html). Wir geben alle getesteten Spezies und Anwendungen auf unseren Produktdatenblättern an und aktualisieren diese sobald wir neue Informationen erhalten. Falls sich herausstellt, dass der Antikörper nicht so funktioniert, wie auf dem Datenblatt beschrieben und Sie sich innerhalb von sechs Monaten mit Ihrem Problem an uns wenden, werden wir Ihnen gerne Vorschläge zur Optimierung Ihres Protokolls oder gegebenenfalls einen Ersatz oder eine Gutschrift schicken. Falls der Antikörper in einer bisher nicht getesteten Anwendung oder Spezies verwendet wird (z.B. für ab111855 oder ab115855), qualifizieren Sie sich für unser Testprogramm: Bei diesem Angebot bekommen Sie einen 100%igen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden. Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen: https://www.abcam.com/collaborationdiscount. Falls Sie daran interessiert sind oder weitere Fragen haben, kontaktieren Sie bitte unseren wissenschaftlichen Dienst. Die oben genannten Antikörper sind nur eine Auswahl und wir haben noch weitere gegen die entsprechenden Zielproteine erhältlich, die für unterschiedliche Anwendungen getestet und garantiert sind. Bei einer detaillierteren Suche bzw. Beratung bin ich Ihnen gerne weiter behilflich. Gerne senden wir Ihnen auch gerne ein Angebot zu, sobald Sie eine konkrete Auswahl getroffen haben. Ich hoffe, diese Informationen helfen Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question

I would like to test this antibody in rat.

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Answer

Thank you very much for your interest in ab795. To our knowledge, ab795 has not been tested in rat. Therefore, I can offer a discount off a future purchase if you buy ab795 now, test it in rat and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab795 in rat. I will then send a discount code. This code must be issued before purchasing ab795 so please wait for my reply before ordering. 2. Purchase ab795 either by phone, fax, or online (www.abcam.com). 3. Test it in rat. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab795 turns out to be unsuitable for rat you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.  

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Question

I am interested in purchasing your NCF1 antibody (ab795). Do you have any information regarding its suitability for immunoprecipitation techniques.

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Answer

Thank you for your enquiry. To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will forward a reward of your choice, typically an Amazon gift voucher.

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