Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13134-23] to NCF1/p47-phox
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Product nameAnti-NCF1/p47-phox antibody [EPR13134-23]
See all NCF1/p47-phox primary antibodies
DescriptionRabbit monoclonal [EPR13134-23] to NCF1/p47-phox
Tested applicationsSuitable for: WB, IHC-P, IPmore details
Species reactivityReacts with: Human
Recombinant fragment within Human NCF1/p47-phox aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: P14598
- Raji and Ramos cell lysates; Human spleen tissue.
Previously labelled as NCF1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Involvement in diseaseDefects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Sequence similaritiesContains 1 PX (phox homology) domain.
Contains 2 SH3 domains.
- Information by UniProt
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
All lanes : Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090) at 1/2000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Western blot analysis of Raji cell lysate immunoprecipitated with ab181090 at 1/20 dilution (Lane 1). Lane 2: Negative control.
Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling NCF1/p47-phox with ab181090 at 1/500 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
ab181090 has not yet been referenced specifically in any publications.