For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    ncf1p47-phox-antibody-epr13134-ab179457.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Metabolism Energy Metabolism
Share by email
RecombinantRabMAb

Recombinant Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)

  • Datasheet
  • SDS
Reviews (1) Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
  • Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
  • Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13134] to NCF1/p47-phox
  • Suitable for: WB, IP
  • Reacts with: Human

You may also be interested in

Primary
Product image
Anti-NOXA2/p67phox antibody [EPR5065] (ab109523)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Primary
Product image
Anti-Annexin A1/ANXA1 antibody [EPR19342] - BSA and Azide free (ab222398)

View more associated products

Overview

  • Product name

    Anti-NCF1/p47-phox antibody [EPR13134]
    See all NCF1/p47-phox primary antibodies
  • Description

    Rabbit monoclonal [EPR13134] to NCF1/p47-phox
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NCF1/p47-phox. The exact sequence is proprietary.
    Database link: P14598

  • Positive control

    • Raji, Ramos and THP1 cell lysates; Raji cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as NCF1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR13134
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Metabolism
    • Energy Metabolism
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Energy Metabolism

Associated products

  • Alternative Versions

    • Anti-NCF1/p47-phox antibody [EPR13134-23] (ab181090)
    • Anti-NCF1/p47-phox antibody [EPR13134] - BSA and Azide free (ab250099)
    • Anti-NCF1/p47-phox antibody [EPR13134-23] - BSA and Azide free (ab250338)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • Ramos whole cell lysate (ab3955)
    • THP-1 whole cell lysate (ab7913)
  • Recombinant Protein

    • Recombinant Human NCF1/p47-phox protein (ab98054)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab179457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
    • Involvement in disease

      Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
    • Sequence similarities

      Contains 1 PX (phox homology) domain.
      Contains 2 SH3 domains.
    • Cellular localization

      Cytoplasm.
    • Target information above from: UniProt accession P14598 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 653361 Human
      • Omim: 608512 Human
      • SwissProt: P14598 Human
      • Unigene: 647047 Human
      • Unigene: 655201 Human
      • Alternative names

        • 47 kDa autosomal chronic granulomatous disease protein antibody
        • 47 kDa neutrophil oxidase factor antibody
        • NADPH oxidase organizer 2 antibody
        • NCF 47K antibody
        • NCF-1 antibody
        • NCF-47K antibody
        • Ncf1 antibody
        • NCF1_HUMAN antibody
        • Neutrophil cytosol factor 1 antibody
        • Neutrophil cytosolic factor 1 antibody
        • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
        • Neutrophil NADPH oxidase factor 1 antibody
        • Nox organizer 2 antibody
        • Nox organizing protein 2 antibody
        • Nox-organizing protein 2 antibody
        • NOXO2 antibody
        • p47 phox antibody
        • p47-phox antibody
        • SH3 and PX domain containing protein 1A antibody
        • SH3 and PX domain-containing protein 1A antibody
        • SH3PXD1A antibody
        see all

      Images

      • Western blot - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
        Western blot - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
        All lanes : Anti-NCF1/p47-phox antibody [EPR13134] (ab179457) at 1/1000 dilution

        Lane 1 : Raji cell lysate
        Lane 2 : Ramos cell lysate
        Lane 3 : THP1 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 45 kDa

      • Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
        Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)

        Western blot analysis on Immunoprecipitation pellet from either 1) Raji cell lysate, or 2) 1xPBS (negative control); showing NCF1/p47-phox, using ab179457 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      • Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
        Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)

      Protocols

      • Western blot protocols
      • Immunoprecipitation protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab179457? Please let us know so that we can cite the reference in this datasheet.

      ab179457 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-NCF1/p47-phox antibody [EPR13134]

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (muscle)
      Gel Running Conditions
      Reduced Denaturing (9)
      Loading amount
      20 µg
      Specification
      muscle
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Mr. Baptiste Jude

      Verified customer

      Submitted Jul 18 2016

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.