Recombinant Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
![Western blot - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)](https://www.abcam.com/ps/products/179/ab179457/Images/ab179457-207492-anti-ncf1-p47-phox-antibody-epr13134-western-blot.jpg "Western blot - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13134] to NCF1/p47-phox
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-NCF1/p47-phox antibody [EPR13134]
See all NCF1/p47-phox primary antibodies -
Description
Rabbit monoclonal [EPR13134] to NCF1/p47-phox -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human NCF1/p47-phox. The exact sequence is proprietary.
Database link: P14598 -
Positive control
- Raji, Ramos and THP1 cell lysates; Raji cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as NCF1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13134 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 45 kDa. |
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IP
1/10 - 1/100. |
Target
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Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
Involvement in disease
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
Sequence similarities
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 653361 Human
- Omim: 608512 Human
- SwissProt: P14598 Human
- Unigene: 647047 Human
- Unigene: 655201 Human
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Alternative names
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
see all
Images
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Western blot - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)All lanes : Anti-NCF1/p47-phox antibody [EPR13134] (ab179457) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa -
![Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)](https://www.abcam.com/ps/products/179/ab179457/Images/ab179457-207491-anti-ncf1-p47-phox-antibody-epr13134-immunoprecipitation.jpg)
Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)Western blot analysis on Immunoprecipitation pellet from either 1) Raji cell lysate, or 2) 1xPBS (negative control); showing NCF1/p47-phox, using ab179457 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)](https://www.abcam.com/ps/products/179/ab179457/Images/ab179457-4-benefits-of-recombinant-antibodies.png)
Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab179457 has not yet been referenced specifically in any publications.