Recombinant
RabMAb

Recombinant Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)

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Overview

  • Product name

    Anti-NCF1/p47-phox antibody [EPR13134]
    See all NCF1/p47-phox primary antibodies

  • Description

    Rabbit monoclonal [EPR13134] to NCF1/p47-phox

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human NCF1/p47-phox. The exact sequence is proprietary.
    Database link: P14598

  • Positive control

    • Raji, Ramos and THP1 cell lysates; Raji cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Previously labelled as NCF1.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab179457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

    • Involvement in disease

      Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

    • Sequence similarities

      Contains 1 PX (phox homology) domain.
      Contains 2 SH3 domains.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • 47 kDa autosomal chronic granulomatous disease protein antibody
        • 47 kDa neutrophil oxidase factor antibody
        • NADPH oxidase organizer 2 antibody
        • NCF 47K antibody
        • NCF-1 antibody
        • NCF-47K antibody
        • Ncf1 antibody
        • NCF1_HUMAN antibody
        • Neutrophil cytosol factor 1 antibody
        • Neutrophil cytosolic factor 1 antibody
        • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
        • Neutrophil NADPH oxidase factor 1 antibody
        • Nox organizer 2 antibody
        • Nox organizing protein 2 antibody
        • Nox-organizing protein 2 antibody
        • NOXO2 antibody
        • p47 phox antibody
        • p47-phox antibody
        • SH3 and PX domain containing protein 1A antibody
        • SH3 and PX domain-containing protein 1A antibody
        • SH3PXD1A antibody
        see all

      Images

      • Western blot - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
        Western blot - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
        All lanes : Anti-NCF1/p47-phox antibody [EPR13134] (ab179457) at 1/1000 dilution

        Lane 1 : Raji cell lysate
        Lane 2 : Ramos cell lysate
        Lane 3 : THP1 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution

        Developed using the ECL technique.

        Predicted band size: 45 kDa

      • Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)
        Immunoprecipitation - Anti-NCF1/p47-phox antibody [EPR13134] (ab179457)

        Western blot analysis on Immunoprecipitation pellet from either 1) Raji cell lysate, or 2) 1xPBS (negative control); showing NCF1/p47-phox, using ab179457 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      References

      ab179457 has not yet been referenced specifically in any publications.

      Publishing research using ab179457? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-NCF1/p47-phox antibody [EPR13134]

       Poor
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - whole (muscle)
      Gel Running Conditions
      Reduced Denaturing (9)
      Loading amount
      20 µg
      Specification
      muscle
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Mr. Baptiste Jude

      Verified customer

      Submitted Jul 18 2016

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