Product nameAnti-NCF1/p47-phox antibody [EPR13134]
See all NCF1/p47-phox primary antibodies
DescriptionRabbit monoclonal [EPR13134] to NCF1/p47-phox
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Human
Recombinant fragment within Human NCF1/p47-phox. The exact sequence is proprietary.
Database link: P14598
- Raji, Ramos and THP1 cell lysates; Raji cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Previously labelled as NCF1.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab179457 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 45 kDa.|
|IP||1/10 - 1/100.|
FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Involvement in diseaseDefects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Sequence similaritiesContains 1 PX (phox homology) domain.
Contains 2 SH3 domains.
- Information by UniProt
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
All lanes : Anti-NCF1/p47-phox antibody [EPR13134] (ab179457) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Ramos cell lysate
Lane 3 : THP1 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Standard HRP labeled goat anti-rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Western blot analysis on Immunoprecipitation pellet from either 1) Raji cell lysate, or 2) 1xPBS (negative control); showing NCF1/p47-phox, using ab179457 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab179457 has not yet been referenced specifically in any publications.