Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)
Key features and details
- Rabbit polyclonal to NCF1/p47-phox (phospho S304)
- Suitable for: WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-NCF1/p47-phox (phospho S304) antibody
See all NCF1/p47-phox primary antibodies -
Description
Rabbit polyclonal to NCF1/p47-phox (phospho S304) -
Host species
Rabbit -
Specificity
Detects NCF1/p47-phox only when phosphorylated at serine 304.
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Tested Applications & Species
Application Species WB African green monkey -
Immunogen
Synthetic peptide corresponding to Human NCF1/p47-phox (phospho S304).
Sequence:R-S-Sp-I-R
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Positive control
- COS7 cells
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General notes
Previously labelled as NCF1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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WB |
African green monkey
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Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
Involvement in disease
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
Sequence similarities
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 653361 Human
- Omim: 608512 Human
- SwissProt: P14598 Human
- Unigene: 647047 Human
- Unigene: 655201 Human
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Alternative names
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
see all
Images
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All lanes : Anti-NCF1/p47-phox (phospho S304) antibody (ab63554) at 1/500 dilution
Lane 1 : extracts from COS7 cells treated
with UV (15mins)
Lane 2 : A549 cells extracts
Lane 3 : A549 cells extracts with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab63554 has been referenced in 2 publications.
- Sowrirajan B et al. Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47(phox). Sci Rep 7:43441 (2017). PubMed: 28240310
- Leverence JT et al. Lipopolysaccharide-induced cytokine expression in alveolar epithelial cells: Role of PKC?-mediated p47phox phosphorylation. Chem Biol Interact : (2010). WB ; Human . PubMed: 20920494