Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)

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Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)

    Key features and details

    • Rabbit polyclonal to NCF1/p47-phox (phospho S304)
    • Suitable for: WB
    • Reacts with: Human, African green monkey
    • Isotype: IgG

    Overview

    • Product name

      Anti-NCF1/p47-phox (phospho S304) antibody
      See all NCF1/p47-phox primary antibodies

    • Description

      Rabbit polyclonal to NCF1/p47-phox (phospho S304)

    • Host species

      Rabbit

    • Specificity

      Detects NCF1/p47-phox only when phosphorylated at serine 304.

    • Tested Applications & Species

      Application Species
      WB
      African green monkey
      See all applications and species data

    • Immunogen

      Synthetic peptide corresponding to Human NCF1/p47-phox (phospho S304).
      Sequence:

      R-S-Sp-I-R

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • COS7 cells

    • General notes

      Previously labelled as NCF1. 

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab63554 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    African green monkey
    Application Abreviews Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
    Notes
    WB
    1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

    Target

    • Function

      NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

    • Involvement in disease

      Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.

    • Sequence similarities

      Contains 1 PX (phox homology) domain.
      Contains 2 SH3 domains.

    • Cellular localization

      Cytoplasm.
    • Information by UniProt

    • Database links

      • Alternative names

        • 47 kDa autosomal chronic granulomatous disease protein antibody
        • 47 kDa neutrophil oxidase factor antibody
        • NADPH oxidase organizer 2 antibody
        • NCF 47K antibody
        • NCF-1 antibody
        • NCF-47K antibody
        • Ncf1 antibody
        • NCF1_HUMAN antibody
        • Neutrophil cytosol factor 1 antibody
        • Neutrophil cytosolic factor 1 antibody
        • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
        • Neutrophil NADPH oxidase factor 1 antibody
        • Nox organizer 2 antibody
        • Nox organizing protein 2 antibody
        • Nox-organizing protein 2 antibody
        • NOXO2 antibody
        • p47 phox antibody
        • p47-phox antibody
        • SH3 and PX domain containing protein 1A antibody
        • SH3 and PX domain-containing protein 1A antibody
        • SH3PXD1A antibody
        see all

      Images

      • Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)
        Western blot - Anti-NCF1/p47-phox (phospho S304) antibody (ab63554)
        All lanes : Anti-NCF1/p47-phox (phospho S304) antibody (ab63554) at 1/500 dilution

        Lane 1 : extracts from COS7 cells treated
        with UV (15mins)
        Lane 2 : A549 cells extracts
        Lane 3 : A549 cells extracts with immunizing peptide at 10 µg

        Lysates/proteins at 30 µg per lane.

        Predicted band size: 45 kDa
        Observed band size: 45 kDa

      References (2)

      Publishing research using ab63554? Please let us know so that we can cite the reference in this datasheet.

      ab63554 has been referenced in 2 publications.

      • Sowrirajan B  et al. Interleukin-27 Enhances the Potential of Reactive Oxygen Species Generation from Monocyte-derived Macrophages and Dendritic cells by Induction of p47(phox). Sci Rep 7:43441 (2017). PubMed: 28240310
      • Leverence JT  et al. Lipopolysaccharide-induced cytokine expression in alveolar epithelial cells: Role of PKC?-mediated p47phox phosphorylation. Chem Biol Interact : (2010). WB ; Human . PubMed: 20920494

      Customer reviews and Q&As

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      1-3 of 3 Abreviews or Q&A

      Question

      Would like to use antibody in Flow Cytometry.

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      Answer

      DISCOUNT CODE: XXXXXXX
      Expiration date: 31-05-2013
      Value: £235

      I am very pleased to hear you would like to accept our offer and test ab63554 in Flow Cytometry. This code will give you ab63554 off your next order before the expiration date. To redeem this offer, please submit an Abreview for Flow Cytometry and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
      For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/abtrial.

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      Question

      Are there any antibodies available that detect the phosphorylated form of p47phox (a regulatory NADPH oxidase subunit) in mouse tissue? I have attached a recent paper which has published in antioxidants and redox signaling. Authors used an antibody from your company for the detection of phospho p47phox in cells extracted from humans.



      Thanks,

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      Answer

      Thank you for contacting us.

      Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

      The antibodies we have are; ab74095, ab63331, ab111855, ab115564, ab795, ab63554, ab107125. Please check the datasheets and let me know, if you want me to create a performa invoice for you.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      No signal detected in blots of rat cell lysates; ab74095 gives expected signal with same samples

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      Answer

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ***** for ab74095. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.  

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