Key features and details
- Rabbit polyclonal to NCF1/p47-phox (phospho S359)
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NCF1/p47-phox (phospho S359) antibody
See all NCF1/p47-phox primary antibodies
DescriptionRabbit polyclonal to NCF1/p47-phox (phospho S359)
ab74095 detects endogenous levels of NCF1/p47-phox only when phosphorylated at serine 359.
Tested applicationsSuitable for: WB, ELISA, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
- Human colon carcinoma tissue, HeLa cells and HeLa cell extracts treated with nocodazole (1ug/ml, 18hours) or TSA (400nM, 24hours).
Previously labelled as NCF1.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab74095 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Our Abpromise guarantee covers the use of ab74095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/500 - 1/1000.|
FunctionNCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
Involvement in diseaseDefects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
Sequence similaritiesContains 1 PX (phox homology) domain.
Contains 2 SH3 domains.
- Information by UniProt
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
All lanes : Anti-NCF1/p47-phox (phospho S359) antibody (ab74095) at 1/500 dilution
Lane 1 : HeLa cell extracts treated
with nocodazole (1ug/ml, 18hours)
Lane 2 : HeLa cell extracts treated
with TSA (400nM, 24hours)
Lane 3 : HeLa cell extracts treated
with nocodazole (1ug/ml, 18hours) and with TSA (400nM, 24hours) with the immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
ab74095 at 1/50 dilution staining NCF1/p47-phox in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
ab74095 at 1/500 dilution staining NCF1/p47-phox in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
ab74095 has been referenced in 1 publication.
- Fischer MT et al. NADPH oxidase expression in active multiple sclerosis lesions in relation to oxidative tissue damage and mitochondrial injury. Brain 135:886-99 (2012). IHC-P ; Human . PubMed: 22366799