Overview

  • Product name

    Anti-NCF1/p47-phox (phospho S359) antibody
    See all NCF1/p47-phox primary antibodies
  • Description

    Rabbit polyclonal to NCF1/p47-phox (phospho S359)
  • Host species

    Rabbit
  • Specificity

    ab74095 detects endogenous levels of NCF1/p47-phox only when phosphorylated at serine 359.

  • Tested applications

    Suitable for: WB, ELISA, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human NCF1/p47-phox (phospho S359).
    Sequence:

    Q-R-Sp-K-P

  • Positive control

    • Human colon carcinoma tissue, HeLa cells and HeLa cell extracts treated with nocodazole (1ug/ml, 18hours) or TSA (400nM, 24hours).
  • General notes

    Previously labelled as NCF1. 

Properties

Applications

Our Abpromise guarantee covers the use of ab74095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

Target

  • Function

    NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).
  • Involvement in disease

    Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • Sequence similarities

    Contains 1 PX (phox homology) domain.
    Contains 2 SH3 domains.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 47 kDa autosomal chronic granulomatous disease protein antibody
    • 47 kDa neutrophil oxidase factor antibody
    • NADPH oxidase organizer 2 antibody
    • NCF 47K antibody
    • NCF-1 antibody
    • NCF-47K antibody
    • Ncf1 antibody
    • NCF1_HUMAN antibody
    • Neutrophil cytosol factor 1 antibody
    • Neutrophil cytosolic factor 1 antibody
    • neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1) antibody
    • Neutrophil NADPH oxidase factor 1 antibody
    • Nox organizer 2 antibody
    • Nox organizing protein 2 antibody
    • Nox-organizing protein 2 antibody
    • NOXO2 antibody
    • p47 phox antibody
    • p47-phox antibody
    • SH3 and PX domain containing protein 1A antibody
    • SH3 and PX domain-containing protein 1A antibody
    • SH3PXD1A antibody
    see all

Images

  • All lanes : Anti-NCF1/p47-phox (phospho S359) antibody (ab74095) at 1/500 dilution

    Lane 1 : HeLa cell extracts treated
    with nocodazole (1ug/ml, 18hours)
    Lane 2 : HeLa cell extracts treated
    with TSA (400nM, 24hours)
    Lane 3 : HeLa cell extracts treated
    with nocodazole (1ug/ml, 18hours) and with TSA (400nM, 24hours) with the immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • ab74095 at 1/50 dilution staining NCF1/p47-phox in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

  • ab74095 at 1/500 dilution staining NCF1/p47-phox in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

References

This product has been referenced in:

  • Fischer MT  et al. NADPH oxidase expression in active multiple sclerosis lesions in relation to oxidative tissue damage and mitochondrial injury. Brain 135:886-99 (2012). IHC-P ; Human . Read more (PubMed: 22366799) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

Yes we have antibodies to detect the p47PHOX. The common alternative namesfor p47phox is NCF1, NOXO2 and SH3PXD1A.http://www.uniprot.org/uniprot/P14598

The antibodies we have are; ab74095, ab63331, ab111855, ab115564, ab795, ab63554, ab107125. Please check the datasheets and let me know, if you want me to create a performa invoice for you.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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