Anti-NCF1/p47-phox (phospho S359) antibody (ab74095)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-NCF1/p47-phox (phospho S359) antibody
See all NCF1/p47-phox primary antibodies -
Description
Rabbit polyclonal to NCF1/p47-phox (phospho S359) -
Host species
Rabbit -
Specificity
ab74095 detects endogenous levels of NCF1/p47-phox only when phosphorylated at serine 359.
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Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human NCF1/p47-phox (phospho S359).
Sequence:Q-R-Sp-K-P
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Positive control
- Human colon carcinoma tissue, HeLa cells and HeLa cell extracts treated with nocodazole (1ug/ml, 18hours) or TSA (400nM, 24hours).
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General notes
Previously labelled as NCF1.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab74095 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab74095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). | |
ELISA | 1/5000. | |
IHC-P | 1/50 - 1/100. | |
ICC/IF | 1/500 - 1/1000. |
Target
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Function
NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production). -
Involvement in disease
Defects in NCF1 are the cause of chronic granulomatous disease autosomal recessive cytochrome-b-positive type 1 (CGD1) [MIM:233700]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. -
Sequence similarities
Contains 1 PX (phox homology) domain.
Contains 2 SH3 domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 653361 Human
- Omim: 608512 Human
- SwissProt: P14598 Human
- Unigene: 647047 Human
- Unigene: 655201 Human
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Alternative names
- 47 kDa autosomal chronic granulomatous disease protein antibody
- 47 kDa neutrophil oxidase factor antibody
- NADPH oxidase organizer 2 antibody
see all
Images
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All lanes : Anti-NCF1/p47-phox (phospho S359) antibody (ab74095) at 1/500 dilution
Lane 1 : HeLa cell extracts treated
with nocodazole (1ug/ml, 18hours)
Lane 2 : HeLa cell extracts treated
with TSA (400nM, 24hours)
Lane 3 : HeLa cell extracts treated
with nocodazole (1ug/ml, 18hours) and with TSA (400nM, 24hours) with the immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCF1/p47-phox (phospho S359) antibody (ab74095)
ab74095 at 1/50 dilution staining NCF1/p47-phox in human colon carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
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ab74095 at 1/500 dilution staining NCF1/p47-phox in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Fischer MT et al. NADPH oxidase expression in active multiple sclerosis lesions in relation to oxidative tissue damage and mitochondrial injury. Brain 135:886-99 (2012). IHC-P ; Human . Read more (PubMed: 22366799) »