Product nameNCI-H226 whole cell lysate
General notesDescription: Control cell lysates from NCI-H226 cells, human lung cells from squamous cell carcinoma, were cultured in the presence of media containing 10% FCS. Cells were lysed in RIPA buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1 mM EDTA, 1 mM NaF, 1 mM Na3O4, 1 mM PMSF, 1 µg/ml aprotinin, 1 µg/ml leupeptin, 1 µg/ml pepstatin, 1% NP-40, 0.25% sodium deoxycholate). 200 µg of cellular protein was mixed with the same volume of 2 X SDS-PAGE loading buffer.
Growth properties: adherent Morphology: epithelial Organ: lung Disease: squamous cell carcinoma Derived from metastatic site: pleural effusion Isolation date: March, 1980 Gender: Male
FormLyophilised:Add 100 µl distilled water to the sample. Final concentration is 2 mg/ml.
Storage instructionsShipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 5% Glycerol, 1% SDS, 0.002% Bromophenol blue, 31mM Tris HCl, pH 6.8
Concentration information loading...
ab95401 has not yet been referenced specifically in any publications.