Key features and details
- Rabbit polyclonal to Nck-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Nck-2 antibody
See all Nck-2 primary antibodies
DescriptionRabbit polyclonal to Nck-2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human Nck-2 aa 1-380.
MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGY VPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYP ANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWR GSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVV QTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKN YVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALN ERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFH TMDELVEHYKKAPIFTSEHGEKLYLVRALQ
Database link: O43639
- WB: Mouse brain and kidney tissue lysate. IHC-P: Human breast cancer and skin tissue. ICC/IF: HepG2 cells.
Protein previously labeled as Nck beta.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol, PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%
Our Abpromise guarantee covers the use of ab236913 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000.|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionAdapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
Sequence similaritiesContains 1 SH2 domain.
Contains 3 SH3 domains.
Cellular localizationCytoplasm. Endoplasmic reticulum.
- Information by UniProt
- Cytoplasmic protein NCK2 antibody
- GRB4 antibody
- Growth factor receptor bound protein 4 antibody
All lanes : Anti-Nck-2 antibody (ab236913) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Paraffin-embedded human breast cancer tissue stained for Nck-2 using ab236913 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human skin tissue stained for Nck-2 using ab236913 at 1/100 dilution in immunohistochemical analysis.
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for Nck-2 (green) using ab236913 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236913 has not yet been referenced specifically in any publications.