Overview

  • Product name

    Anti-Nck-2 antibody [AF3F11]
    See all Nck-2 primary antibodies
  • Description

    Mouse monoclonal [AF3F11] to Nck-2
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein corresponding to Human Nck-2.

  • Positive control

    • A431 cell lysate.
  • General notes

    Protein previously labeled as Nck beta.

Properties

Applications

Our Abpromise guarantee covers the use of ab80618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IP Use at an assay dependent dilution.

Target

  • Function

    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 SH2 domain.
    Contains 3 SH3 domains.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytoplasmic protein NCK2 antibody
    • GRB4 antibody
    • Growth factor receptor bound protein 4 antibody
    • NCK adaptor protein 2 antibody
    • Nck-2 antibody
    • NCK2 antibody
    • NCK2_HUMAN antibody
    • NCKbeta antibody
    • Non-catalytic region of tyrosine kinase adaptor protein 2 antibody
    • Noncatalytic region of tyrosine kinase beta antibody
    • SH2/SH3 adaptor protein NCK-beta antibody
    see all

Images

  • All lanes : Anti-Nck-2 antibody [AF3F11] (ab80618) at 1/2000 dilution

    Lane 1 : RD Cell lysate
    Lane 2 : 293T Cell lysate
    Lane 3 : PC12 Cell lysate
    Lane 4 : NIH3T3 Cell lysate

    Predicted band size: 43 kDa



    Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

  • All lanes : Anti-Nck-2 antibody [AF3F11] (ab80618) at 1/2000 dilution

    Lane 1 : NCK 1
    Lane 2 : NCK beta

    Predicted band size: 43 kDa



    Ladder M.W(kDa): 7, 17, 25, 30, 46, 58, 80, 175

  • All lanes : Anti-Nck-2 antibody [AF3F11] (ab80618) at 1/2000 dilution

    Lane 1 : U937 cell lysate
    Lane 2 : Hepg2 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : SK-N-MC cell lysate

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

References

ab80618 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry regarding ab80618.

I can confirm that the cross-reactivity of this antibody has been tested in Rat and Human samples. Other species has not been determined yet. For further information, please could you advise your customer to consult the Western blot image and the legend (detailedlist of the cell lysates used) on the on-line product datasheet.

If you need any further assistance in the future, please do not hesitate to contact me.

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