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Cell Biology Cell Cycle Cell Cycle Inhibitors Other
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RecombinantRabMAb

Recombinant Anti-Nck-2 antibody [EPR3333] (ab109239)

  • Datasheet
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Abpromise

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Find out more.

Western blot - Anti-Nck-2 antibody [EPR3333] (ab109239)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck-2 antibody [EPR3333] (ab109239)
  • Immunocytochemistry/ Immunofluorescence - Anti-Nck-2 antibody [EPR3333] (ab109239)
  • Anti-Nck-2 antibody [EPR3333] (ab109239)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3333] to Nck-2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-Nck-2 antibody [EPR3333]
    See all Nck-2 primary antibodies
  • Description

    Rabbit monoclonal [EPR3333] to Nck-2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Nck-2 aa 50-150. The exact sequence is proprietary.

  • Positive control

    • Human spleen lysate, human fetal brain lysate, SH-SY5Y lysate, Human fetal kidney lysate; Human breast ductal carcinoma tissue; HeLa cells
  • General notes

     

     

    Protein previously labeled as Nck beta.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3333
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • Other
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Actin Assembly
    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Proto-oncogenes
    • Cancer
    • Cell cycle
    • Cell cycle inhibitors
    • Other
    • Cancer
    • Signal transduction
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Other

Associated products

  • Alternative Versions

    • Anti-Nck-2 antibody [EPR3333] - BSA and Azide free (ab247803)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Nck-2 protein (ab167862)

Applications

Our Abpromise guarantee covers the use of ab109239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.

Target

  • Function

    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 1 SH2 domain.
    Contains 3 SH3 domains.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum.
  • Target information above from: UniProt accession O43639 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8440 Human
    • Entrez Gene: 17974 Mouse
    • Entrez Gene: 316369 Rat
    • Omim: 604930 Human
    • SwissProt: O43639 Human
    • SwissProt: O55033 Mouse
    • Unigene: 529244 Human
    • Alternative names

      • Cytoplasmic protein NCK2 antibody
      • GRB4 antibody
      • Growth factor receptor bound protein 4 antibody
      • NCK adaptor protein 2 antibody
      • Nck-2 antibody
      • NCK2 antibody
      • NCK2_HUMAN antibody
      • NCKbeta antibody
      • Non-catalytic region of tyrosine kinase adaptor protein 2 antibody
      • Noncatalytic region of tyrosine kinase beta antibody
      • SH2/SH3 adaptor protein NCK-beta antibody
      see all

    Images

    • Western blot - Anti-Nck-2 antibody [EPR3333] (ab109239)
      Western blot - Anti-Nck-2 antibody [EPR3333] (ab109239)
      All lanes : Anti-Nck-2 antibody [EPR3333] (ab109239) at 1/1000 dilution

      Lane 1 : Human spleen lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : SH-SY5Y lysate
      Lane 4 : Human fetal kidney lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 43 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck-2 antibody [EPR3333] (ab109239)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck-2 antibody [EPR3333] (ab109239)

      Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue using ab109239 at a dilution of 1/50

      Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Nck-2 antibody [EPR3333] (ab109239)
      Immunocytochemistry/ Immunofluorescence - Anti-Nck-2 antibody [EPR3333] (ab109239)
      Immunofluorescent staining of HeLa cells using ab109239 at a dilution of 1/100
    • Anti-Nck-2 antibody [EPR3333] (ab109239)
      Anti-Nck-2 antibody [EPR3333] (ab109239)

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab109239? Please let us know so that we can cite the reference in this datasheet.

    ab109239 has been referenced in 3 publications.

    • Guan T  et al. Aristolochic acid inhibits Slit2-induced migration and tube formation via inactivation of Robo1/Robo2-NCK1/NCK2 signaling pathway in human umbilical vein endothelial cells. Toxicol Lett 300:51-58 (2019). PubMed: 30381256
    • Dubrac A  et al. NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy. Nat Commun 9:3463 (2018). PubMed: 30150707
    • Dubrac A  et al. Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization. Circulation 133:409-21 (2016). PubMed: 26659946

    Customer reviews and Q&As

    Show All Reviews Q&A
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    There are currently no Customer reviews or Questions for ab109239.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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