Recombinant Anti-Nck-2 antibody [EPR3333] (ab109239)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-Nck-2 antibody [EPR3333]
See all Nck-2 primary antibodies -
Description
Rabbit monoclonal [EPR3333] to Nck-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Nck-2 aa 50-150. The exact sequence is proprietary.
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Positive control
- Human spleen lysate, human fetal brain lysate, SH-SY5Y lysate, Human fetal kidney lysate; Human breast ductal carcinoma tissue; HeLa cells
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General notes
Protein previously labeled as Nck beta.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3333 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 43 kDa. | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Function
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 SH2 domain.
Contains 3 SH3 domains. -
Cellular localization
Cytoplasm. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 8440 Human
- Entrez Gene: 17974 Mouse
- Entrez Gene: 316369 Rat
- Omim: 604930 Human
- SwissProt: O43639 Human
- SwissProt: O55033 Mouse
- Unigene: 529244 Human
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Alternative names
- Cytoplasmic protein NCK2 antibody
- GRB4 antibody
- Growth factor receptor bound protein 4 antibody
see all
Images
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![Western blot - Anti-Nck-2 antibody [EPR3333] (ab109239)](https://www.abcam.com/ps/products/109/ab109239/Images/ab109239-121918-anti-nck-2-antibody-epr3333-western-blot.jpg)
Western blot - Anti-Nck-2 antibody [EPR3333] (ab109239)All lanes : Anti-Nck-2 antibody [EPR3333] (ab109239) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Human fetal brain lysate
Lane 3 : SH-SY5Y lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck-2 antibody [EPR3333] (ab109239)](https://www.abcam.com/ps/products/109/ab109239/Images/ab109239-121920-anti-nck-2-antibody-epr3333-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nck-2 antibody [EPR3333] (ab109239)Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue using ab109239 at a dilution of 1/50
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Nck-2 antibody [EPR3333] (ab109239)](https://www.abcam.com/ps/products/109/ab109239/Images/ab109239-121919-anti-nck-2-antibody-epr3333-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Nck-2 antibody [EPR3333] (ab109239)Immunofluorescent staining of HeLa cells using ab109239 at a dilution of 1/100
Protocols
Datasheets and documents
References
This product has been referenced in:
- Guan T et al. Aristolochic acid inhibits Slit2-induced migration and tube formation via inactivation of Robo1/Robo2-NCK1/NCK2 signaling pathway in human umbilical vein endothelial cells. Toxicol Lett 300:51-58 (2019). Read more (PubMed: 30381256) »
- Dubrac A et al. NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy. Nat Commun 9:3463 (2018). Read more (PubMed: 30150707) »
