Overview

  • Product name

    Anti-Nck antibody [5B7]
    See all Nck primary antibodies
  • Description

    Mouse monoclonal [5B7] to Nck
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Monkey
  • Immunogen

    Purified recombinant fragment of Human Nck expressed in E. coli(203-371)

  • Positive control

    • Jurkat, HeLa, HEK293, A431, K562 and COS7 cell lysates; Jurkat cells. Human rectum cancer tissue; Human Nck (aa203-371) recombinant protein.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR133551-6 are from Tissue Culture Supernatant

Properties

Applications

Our Abpromise guarantee covers the use of ab129367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 43 kDa.
ELISA 1/10000.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
  • Sequence similarities

    Contains 1 SH2 domain.
    Contains 3 SH3 domains.
  • Post-translational
    modifications

    Phosphorylated on Ser and Tyr residues.
  • Cellular localization

    Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytoplasmic protein NCK1 antibody
    • Melanoma Nck protein antibody
    • MGC12668 antibody
    • NCK 1 antibody
    • NCK adaptor protein 1 antibody
    • NCK alpha antibody
    • NCK antibody
    • NCK tyrosine kinase antibody
    • Nck-1 antibody
    • NCK1 antibody
    • NCK1_HUMAN antibody
    • NCKalpha antibody
    • Non catalytic region of tyrosine kinase antibody
    • SH2/SH3 adaptor protein NCK alpha antibody
    • SH2/SH3 adaptor protein NCK-alpha antibody
    see all

Images

  • All lanes : Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HEK293 cell lysate
    Lane 4 : A431 cell lysate
    Lane 5 : K562 cell lysate
    Lane 6 : Cos7 cell lysate

    Predicted band size: 43 kDa

  • ab129367 staining Nck antigen by ELISA.
  • ab129367, at 1/200 dilution, staining Nck in Jurkat cells by flow cytometry (green). Negative control shown in red.
  • ab129367, at 1/200 dilution, staining Nck in paraffin-embedded Human rectum cancer tissue by Immunohistochemistry, with DAB staining.
  • Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution + Human Recombinant Nck fragment (aa 203-371)

    Predicted band size: 43 kDa



    The expected molecular weight of the recombinant fragment is 45 kDa.

References

ab129367 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Question
Answer

La concentration d'ab129367 est de 1 mg/ml donc les 100 ul contienent 100 ug d'anticorps.

Read More

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