Anti-Nck antibody [5B7] (ab129367)
Key features and details
- Mouse monoclonal [5B7] to Nck
- Suitable for: WB, ELISA, IHC-P, Flow Cyt
- Reacts with: Human, African green monkey, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-Nck antibody [5B7]
See all Nck primary antibodies -
Description
Mouse monoclonal [5B7] to Nck -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human, African green monkey, Recombinant fragment -
Immunogen
Purified recombinant fragment of Human Nck expressed in E. coli(203-371)
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Positive control
- Jurkat, HeLa, HEK293, A431, K562 and COS7 cell lysates; Jurkat cells. Human rectum cancer tissue; Human Nck (aa203-371) recombinant protein.
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General notes
This product was changed from ascites to supernatant. Lot no’s high than GR133551-6 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5B7 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 43 kDa.
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ELISA |
1/10000.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 43 kDa. |
ELISA
1/10000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. -
Sequence similarities
Contains 1 SH2 domain.
Contains 3 SH3 domains. -
Post-translational
modificationsPhosphorylated on Ser and Tyr residues. -
Cellular localization
Cytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments. - Information by UniProt
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Database links
- Entrez Gene: 4690 Human
- Omim: 600508 Human
- SwissProt: P16333 Human
- Unigene: 477693 Human
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Alternative names
- Cytoplasmic protein NCK1 antibody
- Melanoma Nck protein antibody
- MGC12668 antibody
see all
Images
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All lanes : Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : Cos7 cell lysate
Predicted band size: 43 kDa -
ab129367 staining Nck antigen by ELISA.
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ab129367, at 1/200 dilution, staining Nck in Jurkat cells by flow cytometry (green). Negative control shown in red.
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ab129367, at 1/200 dilution, staining Nck in paraffin-embedded Human rectum cancer tissue by Immunohistochemistry, with DAB staining.
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Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution + Human Recombinant Nck fragment (aa 203-371)
Predicted band size: 43 kDa
The expected molecular weight of the recombinant fragment is 45 kDa.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab129367 has not yet been referenced specifically in any publications.