Key features and details
- Mouse monoclonal [5B7] to Nck
- Suitable for: WB, ELISA, IHC-P, Flow Cyt
- Reacts with: Human, Monkey
- Isotype: IgG1
Product nameAnti-Nck antibody [5B7]
See all Nck primary antibodies
DescriptionMouse monoclonal [5B7] to Nck
Tested applicationsSuitable for: WB, ELISA, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human, Monkey
Purified recombinant fragment of Human Nck expressed in E. coli(203-371)
- Jurkat, HeLa, HEK293, A431, K562 and COS7 cell lysates; Jurkat cells. Human rectum cancer tissue; Human Nck (aa203-371) recombinant protein.
This product was changed from ascites to supernatant. Lot no’s high than GR133551-6 are from Tissue Culture Supernatant
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
- COS7 nuclear extract lysate (ab14643)
- HeLa nuclear extract lysate (ab14655)
- Jurkat cytoplasmic extract lysate (ab14842)
- K562 nuclear extract lysate (ab14851)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab7899)
- HEK293 whole cell lysate (ab7902)
- A431 whole cell lysate (ab7909)
- K562 whole cell lysate (ab7911)
Our Abpromise guarantee covers the use of ab129367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 43 kDa.|
|IHC-P||1/200 - 1/1000.|
|Flow Cyt||1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionAdapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2.
Sequence similaritiesContains 1 SH2 domain.
Contains 3 SH3 domains.
modificationsPhosphorylated on Ser and Tyr residues.
Cellular localizationCytoplasm. Endoplasmic reticulum. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments.
- Information by UniProt
- Cytoplasmic protein NCK1 antibody
- Melanoma Nck protein antibody
- MGC12668 antibody
All lanes : Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : A431 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : Cos7 cell lysate
Predicted band size: 43 kDa
ab129367 staining Nck antigen by ELISA.
ab129367, at 1/200 dilution, staining Nck in Jurkat cells by flow cytometry (green). Negative control shown in red.
ab129367, at 1/200 dilution, staining Nck in paraffin-embedded Human rectum cancer tissue by Immunohistochemistry, with DAB staining.
Anti-Nck antibody [5B7] (ab129367) at 1/500 dilution + Human Recombinant Nck fragment (aa 203-371)
Predicted band size: 43 kDa
The expected molecular weight of the recombinant fragment is 45 kDa.
ab129367 has not yet been referenced specifically in any publications.