Recombinant Anti-Nck-2 antibody [EPR3333] (ab109239)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3333] to Nck-2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Nck-2 antibody [EPR3333]
See all Nck-2 primary antibodies -
Description
Rabbit monoclonal [EPR3333] to Nck-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human spleen lysate, human fetal brain lysate, SH-SY5Y lysate, Human fetal kidney lysate; Human breast ductal carcinoma tissue; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3333 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 43 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/250.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 43 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/250. |
Target
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Function
Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 SH2 domain.
Contains 3 SH3 domains. -
Cellular localization
Cytoplasm. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 8440 Human
- Entrez Gene: 17974 Mouse
- Entrez Gene: 316369 Rat
- Omim: 604930 Human
- SwissProt: O43639 Human
- SwissProt: O55033 Mouse
- Unigene: 529244 Human
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Alternative names
- Cytoplasmic protein NCK2 antibody
- GRB4 antibody
- Growth factor receptor bound protein 4 antibody
see all
Images
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All lanes : Anti-Nck-2 antibody [EPR3333] (ab109239) at 1/1000 dilution
Lane 1 : Human spleen lysate
Lane 2 : Human fetal brain tissue lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Human fetal kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa -
Immunohistochemical analysis of paraffin-embedded human breast ductal carcinoma tissue using ab109239 at a dilution of 1/50
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunofluorescent staining of HeLa cells using ab109239 at a dilution of 1/100
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab109239 has been referenced in 5 publications.
- Alfaidi M et al. Nck1, But Not Nck2, Mediates Disturbed Flow-Induced p21-Activated Kinase Activation and Endothelial Permeability. J Am Heart Assoc 9:e016099 (2020). PubMed: 32468886
- Guan T et al. Aristolochic acid inhibits Slit2-induced migration and tube formation via inactivation of Robo1/Robo2-NCK1/NCK2 signaling pathway in human umbilical vein endothelial cells. Toxicol Lett 300:51-58 (2019). PubMed: 30381256
- Dubrac A et al. NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy. Nat Commun 9:3463 (2018). PubMed: 30150707
- Dubrac A et al. Targeting NCK-Mediated Endothelial Cell Front-Rear Polarity Inhibits Neovascularization. Circulation 133:409-21 (2016). PubMed: 26659946
- Tie SR et al. Regulation of sarcoma cell migration, invasion and invadopodia formation by AFAP1L1 through a phosphotyrosine-dependent pathway. Oncogene 35:2098-111 (2016). PubMed: 26212012