Product nameNck overexpression 293T lysate (whole cell)
ab94184 is a 293T cell transfected lysate in which Human Nck has been transiently over-expressed using a pCMV-Nck plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferpH: 6.80
Constituent: 100% 1x Sample Buffer
Concentration information loading...
BackgroundFunction: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Similarity: Contains 1 SH2 domain. Contains 3 SH3 domains. PTM: Phosphorylated on Ser and Tyr residues.
Our Abpromise guarantee covers the use of ab94184 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94184 at 15 μg/lane on an SDS-PAGE gel
All lanes : Anti-Nck antibody (ab55499) at 1/500 dilution
Lane 1 :
Nck overexpression 293T lysate (whole cell) (ab94184)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94184 has not yet been referenced specifically in any publications.