Overview

  • Product name

  • Description

    Rabbit polyclonal to NCKAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Xenopus laevis, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within N terminal amino acids 2-298 of Human NCKAP1 (Q9Y2A7).

  • Positive control

    • NT2D1, IMR32, U-87 MG, MCF7 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab126061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 128 kDa.

Target

  • Function

    Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.
  • Tissue specificity

    Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
  • Sequence similarities

    Belongs to the HEM-1/HEM-2 family.
  • Cellular localization

    Cell membrane. Cell projection > lamellipodium membrane. At the interface between the lamellipodial actin meshwork and the membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ11291 antibody
    • KIAA0587 antibody
    • Membrane associated protein HEM2 antibody
    • Membrane-associated protein HEM-2 antibody
    • MGC8981 antibody
    • NAP 1 antibody
    • NAP1 antibody
    • NAP125 antibody
    • NCK associated protein 1 antibody
    • NCK associated protein antibody
    • Nck-associated protein 1 antibody
    • Nckap1 antibody
    • NCKP1_HUMAN antibody
    • OTTHUMP00000163460 antibody
    • OTTHUMP00000163461 antibody
    • p125Nap1 antibody
    see all

Images

  • Anti-NCKAP1 antibody (ab126061) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg

    Predicted band size: 128 kDa



    5% SDS PAGE

References

ab126061 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry.

All the products you have selected have been tested in human samples in western blot. If they have been specifically tested in human fibroblasts this would be stated on the product datasheet. I encourage you to check the images, references and Abreviews to help guide you in making a choice. All the data we have about the product is listed on the datasheet, including immunogen and suggested working dilutions.

Please do not hesitate to contact me should you have questions about specific products and I will do my best to help.

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