Overview

  • Product name

  • Description

    Rabbit polyclonal to NCKAP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide within Human NCKAP1 aa 925-975. The exact sequence is proprietary.
    Database link: Q9Y2A7

  • Positive control

    • IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226303 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • NCKAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer: 1 mg for IP, 20% of IP loaded) with ab226303 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226303 at 0.4 µg/ml.

      Lane 1: ab226303 IP in HEK-293T whole cell lysate.

      Lane 2: Control IgG IP in HEK-293T whole cell lysate.

      Detection:  Chemiluminescence with exposure time of 10 seconds.

    References

    ab226303 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab226303.
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