Overview

  • Product name

    Anti-NCKAP1 antibody - N-terminal
    See all NCKAP1 primary antibodies
  • Description

    Rabbit polyclonal to NCKAP1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human NCKAP1 aa 1-50 (N terminal). The exact sequence is proprietary.
    Database link: Q9Y2A7

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226302 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 128 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.
  • Tissue specificity

    Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
  • Sequence similarities

    Belongs to the HEM-1/HEM-2 family.
  • Cellular localization

    Cell membrane. Cell projection > lamellipodium membrane. At the interface between the lamellipodial actin meshwork and the membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ11291 antibody
    • KIAA0587 antibody
    • Membrane associated protein HEM2 antibody
    • Membrane-associated protein HEM-2 antibody
    • MGC8981 antibody
    • NAP 1 antibody
    • NAP1 antibody
    • NAP125 antibody
    • NCK associated protein 1 antibody
    • NCK associated protein antibody
    • Nck-associated protein 1 antibody
    • Nckap1 antibody
    • NCKP1_HUMAN antibody
    • OTTHUMP00000163460 antibody
    • OTTHUMP00000163461 antibody
    • p125Nap1 antibody
    see all

Images

  • All lanes : Anti-NCKAP1 antibody - N-terminal (ab226302) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 128 kDa


    Exposure time: 10 seconds


    Lysates prepared using NETN lysis buffer.

  • NCKAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226302 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226302 at 0.4 µg/ml.

    Lane 1: ab226302 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 10 seconds.

References

ab226302 has not yet been referenced specifically in any publications.

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