Key features and details
- Rabbit polyclonal to NCKAP1 - N-terminal
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NCKAP1 antibody - N-terminal
See all NCKAP1 primary antibodies
DescriptionRabbit polyclonal to NCKAP1 - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human NCKAP1 aa 1-50 (N terminal). The exact sequence is proprietary.
Database link: Q9Y2A7
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226302 was affinity purified using an epitope specific to NCKAP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 128 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPart of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1.
Tissue specificityExpressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.
Sequence similaritiesBelongs to the HEM-1/HEM-2 family.
Cellular localizationCell membrane. Cell projection > lamellipodium membrane. At the interface between the lamellipodial actin meshwork and the membrane.
- Information by UniProt
- FLJ11291 antibody
- KIAA0587 antibody
- Membrane associated protein HEM2 antibody
All lanes : Anti-NCKAP1 antibody - N-terminal (ab226302) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 128 kDa
Exposure time: 10 seconds
Lysates prepared using NETN lysis buffer.
NCKAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) with ab226302 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab226302 at 0.4 µg/ml.
Lane 1: ab226302 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226302 has not yet been referenced specifically in any publications.