Key features and details
- Rabbit polyclonal to NCKAP1L
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NCKAP1L antibody
See all NCKAP1L primary antibodies
DescriptionRabbit polyclonal to NCKAP1L
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
- WB: RT4, U-251MG sp, Human plasma (IgG/HSA depleted), Human liver tissue and tonsil tissue. IHC-P: Human lymph tissue, small intestine tissue, liver tissue, lung tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab272631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
Tissue specificityExpressed only in cells of hematopoietic origin.
Sequence similaritiesBelongs to the HEM-1/HEM-2 family.
Cellular localizationCell membrane.
- Information by UniProt
- 4930568P13Rik antibody
- AI463083 antibody
- HEM1 antibody
Lanes 2-6 : Anti-NCKAP1L antibody (ab272631) at 0.4 µg/ml
Lane 1 : Molecular weight ladder
Lane 2 : RT4
Lane 3 : U-251MG sp
Lane 4 : uman plasma (IgG/HSA depleted)
Lane 5 : Human liver tissue
Lane 6 : Human tonsil tissue
Paraffin-embedded formalin-fixed Human lymph tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
Paraffin-embedded formalin-fixed Human small intestine tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
Paraffin-embedded formalin-fixed Human liver tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
Paraffin-embedded formalin-fixed Human lung tissue stained for NCKAP1L using ab272631 at 1/500 in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab272631 has not yet been referenced specifically in any publications.