Anti-NCOA4 antibody [439CT10.1.2] (ab62495)


  • Product name
    Anti-NCOA4 antibody [439CT10.1.2]
    See all NCOA4 primary antibodies
  • Description
    Mouse monoclonal [439CT10.1.2] to NCOA4
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant protein fragment derived from within C-terminal residues of Human NCOA4.

  • Positive control
    • This antibody gave a positive signal in HEK293 and PC3 whole cell lysates.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact or you can find further information here.



Our Abpromise guarantee covers the use of ab62495 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). Use 3% milk as blocking agent.


  • Function
    Enhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.
  • Tissue specificity
    Widely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis.
  • Involvement in disease
    Defects in NCOA4 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas.
  • Information by UniProt
  • Database links
  • Alternative names
    • 70 kDa androgen receptor activator antibody
    • 70 kDa androgen receptor coactivator antibody
    • 70 kDa AR activator antibody
    • 70 kDa AR-activator antibody
    • Androgen receptor coactivator 70 kD antibody
    • Androgen receptor coactivator 70 kDa protein antibody
    • Androgen receptor-associated protein of 70 kDa antibody
    • ARA 70 antibody
    • ARA70 antibody
    • DKFZp762E1112 antibody
    • ELE 1 antibody
    • ELE1 antibody
    • ELE1/ret TK antibody
    • NCOA 4 antibody
    • NCoA-4 antibody
    • NCOA4 antibody
    • NCOA4_HUMAN antibody
    • Nuclear receptor coactivator 4 antibody
    • Papillary thyroid carcinoma 3 antibody
    • PTC 3 antibody
    • PTC3 antibody
    • RET activating gene ELE1 antibody
    • Ret activating protein ELE1 antibody
    • Ret-activating protein ELE1 antibody
    • RFG antibody
    see all


  • All lanes : Anti-NCOA4 antibody [439CT10.1.2] (ab62495) at 1/100 dilution

    Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa
    Observed band size: 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 16 minutes

    Abcam recommends using 3% milk as the blocking agent.


ab62495 has not yet been referenced specifically in any publications.

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