Key features and details
- Mouse monoclonal [439CT10.1.2] to NCOA4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgM
Product nameAnti-NCOA4 antibody [439CT10.1.2]
See all NCOA4 primary antibodies
DescriptionMouse monoclonal [439CT10.1.2] to NCOA4
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human NCOA4 aa 400 to the C-terminus (C terminal).
- This antibody gave a positive signal in HEK293 and PC3 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityConcentrated Culture Supernatant
Our Abpromise guarantee covers the use of ab62495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Detects a band of approximately 75 kDa (predicted molecular weight: 70 kDa). Use 3% milk as blocking agent.|
FunctionEnhances the androgen receptor transcriptional activity in prostate cancer cells. Ligand-independent coactivator of the peroxisome proliferator-activated receptor (PPAR) gamma.
Tissue specificityWidely expressed. Also detected in adipose tissues and in different cell lines. Isoform Beta is only expressed in testis.
Involvement in diseaseDefects in NCOA4 are a cause of thyroid papillary carcinoma (TPC) [MIM:188550]. TPC is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue. Papillary carcinomas are malignant neoplasm characterized by the formation of numerous, irregular, finger-like projections of fibrous stroma that is covered with a surface layer of neoplastic epithelial cells. Note=A chromosomal aberration involving NCOA4 is found in thyroid papillary carcinomas. Inversion inv(10)(q11.2;q11.2) generates the RET/NCOA4 (PTC3) oncogene that has been found in sporadic and radiation-associated post-Chernobyl thyroid papillary carcinomas.
- Information by UniProt
- 70 kDa androgen receptor activator antibody
- 70 kDa androgen receptor coactivator antibody
- 70 kDa AR activator antibody
All lanes : Anti-NCOA4 antibody [439CT10.1.2] (ab62495) at 1/100 dilution
Lane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 2 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Peroxidase- conjugated AffiniPure Goat Anti-mouse IgM at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes
Abcam recommends using 3% milk as the blocking agent.
ab62495 has not yet been referenced specifically in any publications.