Product nameAnti-NCOA62 antibody [EPR9989(B)]
See all NCOA62 primary antibodies
DescriptionRabbit monoclonal [EPR9989(B)] to NCOA62
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide corresponding to residues in Human NCOA62 (UniProt Q13573).
- HeLa, A431, Raw264.7, NIH/3T3 and 293T cell lysates; A431 cells.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab167165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 61 kDa.|
|ICC/IF||1/100 - 1/250.|
RelevanceNuclear receptor coactivator NCoA62 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.
- Bx 42 antibody
- Bx42 antibody
- Homolog of Drosophila BX42 antibody
All lanes : Anti-NCOA62 antibody [EPR9989(B)] (ab167165) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : Raw264.7 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 61 kDa
Immunofluorescent analysis of A431 cells labeling NCOA62 with ab167165 at 1/100 dilution.
ab167165 has not yet been referenced specifically in any publications.