Anti-NCOA62/SNW1 antibody (ab227221)
Key features and details
- Rabbit polyclonal to NCOA62/SNW1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NCOA62/SNW1 antibody
See all NCOA62/SNW1 primary antibodies -
Description
Rabbit polyclonal to NCOA62/SNW1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Chimpanzee
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Immunogen
Recombinant fragment within Human NCOA62/SNW1 (internal sequence). The exact sequence is proprietary.
Database link: Q13573 -
Positive control
- WB: A549 and HepG2 whole cell lysates. IHC-P: Human ovarian cancer tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as NCOA62
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Relevance
Nuclear receptor coactivator NCOA62/SNW1 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 326578 Cow
- Entrez Gene: 22938 Human
- Entrez Gene: 66354 Mouse
- Entrez Gene: 500695 Rat
- Omim: 603055 Human
- SwissProt: Q1JQE0 Cow
- SwissProt: Q13573 Human
- SwissProt: Q9CSN1 Mouse
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Alternative names
- Bx 42 antibody
- Bx42 antibody
- Homolog of Drosophila BX42 antibody
see all
Images
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Western blot - Anti-NCOA62/SNW1 antibody (ab227221)All lanes : Anti-NCOA62/SNW1 antibody (ab227221) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA62/SNW1 antibody (ab227221)Paraffin-embedded human ovarian cancer tissue stained for NCOA62/SNW1 using ab227221 at 1/250 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NCOA62/SNW1 antibody (ab227221)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NCOA62/SNW1 (green) using ab227221 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab227221 has not yet been referenced specifically in any publications.
