Overview

  • Product name

  • Description

    Rabbit polyclonal to NCOA62/SNW1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Chimpanzee
  • Immunogen

    Recombinant fragment within Human NCOA62/SNW1 (internal sequence). The exact sequence is proprietary.
    Database link: Q13573

  • Positive control

    • WB: A549 and HepG2 whole cell lysates. IHC-P: Human ovarian cancer tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as NCOA62

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227221 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    Nuclear receptor coactivator NCOA62/SNW1 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Bx 42 antibody
    • Bx42 antibody
    • Homolog of Drosophila BX42 antibody
    • MGC119379 antibody
    • NCOA 62 antibody
    • Nuclear protein SkiP antibody
    • Nuclear receptor coactivator 62 kD antibody
    • Nuclear receptor coactivator NCoA 62 antibody
    • Nuclear receptor coactivator NCoA62 antibody
    • Prp 45 antibody
    • Prp45 antibody
    • PRPF 45 antibody
    • PRPF45 antibody
    • Ski interacting protein antibody
    • SKIIP antibody
    • SKIP antibody
    • SNW 1 antibody
    • SNW domain containing 1 antibody
    • SNW domain containing protein 1 antibody
    • SNW1 antibody
    • SNW1 protein antibody
    see all

Images

  • All lanes : Anti-NCOA62/SNW1 antibody (ab227221) at 1/1000 dilution

    Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human ovarian cancer tissue stained for NCOA62/SNW1 using ab227221 at 1/250 dilution in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NCOA62/SNW1 (green) using ab227221 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).

References

ab227221 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227221.
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