Anti-NCOA62/SNW1 antibody (ab227221)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-NCOA62/SNW1 antibody
See all NCOA62/SNW1 primary antibodies -
Description
Rabbit polyclonal to NCOA62/SNW1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Xenopus laevis, Chimpanzee
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Immunogen
Recombinant fragment within Human NCOA62/SNW1 (internal sequence). The exact sequence is proprietary.
Database link: Q13573 -
Positive control
- WB: A549 and HepG2 whole cell lysates. IHC-P: Human ovarian cancer tissue. ICC/IF: HeLa cells.
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General notes
This product was previously labelled as NCOA62
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1% BSA, 20% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 61 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Relevance
Nuclear receptor coactivator NCOA62/SNW1 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 326578 Cow
- Entrez Gene: 22938 Human
- Entrez Gene: 66354 Mouse
- Entrez Gene: 500695 Rat
- Omim: 603055 Human
- SwissProt: Q1JQE0 Cow
- SwissProt: Q13573 Human
- SwissProt: Q9CSN1 Mouse
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Alternative names
- Bx 42 antibody
- Bx42 antibody
- Homolog of Drosophila BX42 antibody
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Images
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Western blot - Anti-NCOA62/SNW1 antibody (ab227221)All lanes : Anti-NCOA62/SNW1 antibody (ab227221) at 1/1000 dilution
Lane 1 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 61 kDa7.5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCOA62/SNW1 antibody (ab227221)Paraffin-embedded human ovarian cancer tissue stained for NCOA62/SNW1 using ab227221 at 1/250 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-NCOA62/SNW1 antibody (ab227221)Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NCOA62/SNW1 (green) using ab227221 at 1/500 dilution in ICC/IF. Nuclear counterstain: Hoechst 33342 (blue).
References
ab227221 has not yet been referenced specifically in any publications.
