Key features and details
- Rabbit polyclonal to NCOA62/SNW1
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NCOA62/SNW1 antibody
See all NCOA62/SNW1 primary antibodies
DescriptionRabbit polyclonal to NCOA62/SNW1
Tested applicationsSuitable for: IP, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
Synthetic peptide corresponding to Human NCOA62/SNW1 aa 450-500.
Database link: NP_036377.1
- HeLa, 293T and NIH3T3 whole cell lysates.
This product was previously labelled as NCOA62
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
RelevanceNuclear receptor coactivator NCOA62/SNW1 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NCOA62/SNW1 with ab70827 at 1/1000 (1µg/ml). Detection: DAB.
All lanes : Anti-NCOA62/SNW1 antibody (ab70827) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 59 kDa
Observed band size: 65 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 40 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Detection of Human NCOA62/SNW1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70827 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
ab70827 has not yet been referenced specifically in any publications.