Overview

  • Product name

  • Description

    Rabbit polyclonal to NCOA62/SNW1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human NCOA62/SNW1 aa 450-500.
    Database link: NP_036377.1

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysates.
  • General notes

     This product was previously labelled as NCOA62

     

Properties

Applications

Our Abpromise guarantee covers the use of ab70827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
WB 1/2000 - 1/10000. Detects a band of approximately 65 kDa (predicted molecular weight: 59 kDa).
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Nuclear receptor coactivator NCOA62/SNW1 (Nuclear Protein SkiP, SKIIP , ski-interacting protein) is a member of the SNW gene family, encodes a coactivator that enhances transcription from some Pol II promoters. This coactivator can bind to the ligand-binding domain of the vitamin D receptor and to retinoid receptors to enhance vitamin D-, retinoic acid-, estrogen-, and glucocorticoid-mediated gene expression. It can also interact with poly(A)-binding protein 2 to directly control the expression of muscle-specific genes at the transcriptional level. Finally, the protein may be involved in oncogenesis since it interacts with a region of SKI oncoproteins that is required for transforming activity.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • Bx 42 antibody
    • Bx42 antibody
    • Homolog of Drosophila BX42 antibody
    • MGC119379 antibody
    • NCOA 62 antibody
    • Nuclear protein SkiP antibody
    • Nuclear receptor coactivator 62 kD antibody
    • Nuclear receptor coactivator NCoA 62 antibody
    • Nuclear receptor coactivator NCoA62 antibody
    • Prp 45 antibody
    • Prp45 antibody
    • PRPF 45 antibody
    • PRPF45 antibody
    • Ski interacting protein antibody
    • SKIIP antibody
    • SKIP antibody
    • SNW 1 antibody
    • SNW domain containing 1 antibody
    • SNW domain containing protein 1 antibody
    • SNW1 antibody
    • SNW1 protein antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling NCOA62/SNW1 with ab70827 at 1/1000 (1µg/ml). Detection: DAB.

  • All lanes : Anti-NCOA62/SNW1 antibody (ab70827) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 59 kDa
    Observed band size: 65 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 28 kDa, 40 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

  • Detection of Human NCOA62/SNW1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70827 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

References

ab70827 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry and your interest in our products.

Do you have any preliminary data regarding the sequence homology between Drosophila and human NCOA62?

According to the Blast search, the homology with Xenopus laevis ( Amphibia) 80% so it is expected to have even lower homology with Drosophila. As you may know, high cross-reactivity can only be expected if the homology is more than 95%.

If you need any further assistance in the future, please do not hesitate to contact me.

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