• Product name

  • Description

    Rabbit polyclonal to NCOR2/SMRT
  • Host species

  • Specificity

    Does not react with NCOR2/SMRT.

  • Tested applications

    Suitable for: ChIP, ICC/IF, IP, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NCOR2/SMRT aa 1165-1363.

  • Positive control

    • HeLa nuclear extract
  • General notes

    Previously labelled as NCOR2.


Our Abpromise guarantee covers the use of ab24551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution.
ICC/IF 1/300.
IP Use at an assay dependent dilution.
WB 1/1000. Detects a band of approximately 274 kDa (predicted molecular weight: 274 kDa).


  • Function

    Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.
  • Tissue specificity

    Ubiquitous. High levels of expression are detected in lung, spleen and brain.
  • Sequence similarities

    Belongs to the N-CoR nuclear receptor corepressors family.
    Contains 2 SANT domains.
  • Domain

    The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
    The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • CTG 26 antibody
    • CTG repeat protein 26 antibody
    • CTG26 antibody
    • N CoR2 antibody
    • N-CoR2 antibody
    • NCoR 2 antibody
    • Ncor2 antibody
    • NCOR2_HUMAN antibody
    • Nuclear Receptor Co-Repressor 2 antibody
    • Nuclear receptor corepressor 2 antibody
    • retinoic-acid-receptor-associated corepressor antibody
    • Silencing Mediator for Retanoid and Thyroid Hormone Receptors antibody
    • Silencing mediator of retinoic acid and thyroid hormone receptor antibody
    • SMAP 270 antibody
    • SMAP270 antibody
    • SMRT antibody
    • SMRTE antibody
    • SMRTE tau antibody
    • T3 receptor associating factor antibody
    • T3 receptor-associating factor antibody
    • Thyroid retinoic acid receptor associated corepressor antibody
    • Thyroid- antibody
    • TNRC 14 antibody
    • TNRC14 antibody
    • TRAC 1 antibody
    • TRAC antibody
    • TRAC1 antibody
    see all


  • Anti-NCOR2/SMRT antibody (ab24551) at 1/1000 dilution + HeLa nuclear extract

    Predicted band size: 274 kDa
    Observed band size: 270 kDa
    why is the actual band size different from the predicted?

  • ab24551 staining NCOR2/SMRT (green) in Human Hela cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Samples were incubated with primary antibody (1/300 in PBS + 0.2% fish scale gelatin) for 20 minutes at 25°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview


This product has been referenced in:

  • Rigalli JP  et al. The pregnane X receptor (PXR) and the nuclear receptor corepressor 2 (NCoR2) modulate cell growth in head and neck squamous cell carcinoma. PLoS One 13:e0193242 (2018). Read more (PubMed: 29470550) »
  • Gong C  et al. BQ323636.1, a Novel Splice Variant to NCOR2, as a Predictor for Tamoxifen-Resistant Breast Cancer. Clin Cancer Res 24:3681-3691 (2018). Read more (PubMed: 29420220) »
See all 18 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (Hela cell)
Hela cell
Blocking step
Fish scale gelatin as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jul 15 2010

Western blot
Human Cell lysate - whole cell (huh-7)
Gel Running Conditions
Reduced Denaturing
Loading amount
15000 cells
Blocking step
Milk as blocking agent for 20 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 10 2008


Thank you for your enquiry. We qualify our "ChIP grade" antibodies in three ways: Through our; 1. In our in-house ChIP testing program, 2. In a scientific publication or 3. by a customer review. On this occasion this antibody was sourced from the authors. We have every confidence in the behaviour of this antibody by ChIP. We make every effort to test as many of our antibodies in house as possible. However, this is not always possible given the cell systems employed to detect the binding of chromatin associated proteins. We only qualify our antibodies as being suitable for chromatin immunoprecipitation following a customers review if we are aware of the protocol used and the necessary optimisation for this antibody. We also monitor the status of our ChIP grade antibodies very closely. Should a ChIP grade antibody be shown to no longer demonstrate the capacity to immunoprecipitate cross linked chromatin we remove ChIP as an application. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More


Thank you for your enquiry. We qualify our antibodies as "ChIP grade" status in three ways 1. in our in-house ChIP testing program, 2. in a scientific publication and 3. by a customer (Abreview). Rabbit polyclonal to NCoR2, ab24551 was qualified as being capable of ChIP by its use in the following publication: Yoon HG et al. Reading and function of a histone code involved in targeting corepressor complexes for repression. Mol Cell Biol 25:324-35 (2005). PubMed: 15601853 In this publication they demonstrated the association of NCoR with the deiodinase 1 (D1) promoter (thyroid hormone response element (TRE)) in a HeLa a2 cell line which constitutively expresses a FLAG-tagged human TRa. They did not use a positive control as such. However, if this does not present itself as a suitable control any thyroid hormone controlled gene that is reactivated with Hdac inhibitors or has been shown to have association with NCoR are good places to start. ab24552 was also qualified by its presence in the same publication. It was used and confirmed by the same authors. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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