Key features and details
- Rabbit polyclonal to NCOR2/SMRT
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NCOR2/SMRT antibody
See all NCOR2/SMRT primary antibodies
DescriptionRabbit polyclonal to NCOR2/SMRT
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human NCOR2/SMRT aa 1925-1975. The exact sequence is proprietary.
Database link: Q9Y618
- IP: HeLa cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Class II / Hda1 Class
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab264188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 2 - 5 µg/ml.|
FunctionTranscriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors.
Tissue specificityUbiquitous. High levels of expression are detected in lung, spleen and brain.
Sequence similaritiesBelongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains.
DomainThe N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity.
- Information by UniProt
- CTG 26 antibody
- CTG repeat protein 26 antibody
- CTG26 antibody
NCOR2/SMRT was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264188 at 3 µg/mg lysate. An alternative NCOR2/SMRT antibody was used for western blotting at 1 µg/ml.
Lane 1: Alternative NCOR2/SMRT antibody IP in HeLa whole cell lysate.
Lane 2: ab264188 IP in HeLa whole cell lysate.
Lane 3: Control IgG.
Detection: Chemiluminescence with an exposure time of 1 second.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264188 has not yet been referenced specifically in any publications.