Anti-NCOR2/SMRT antibody (ab264188)
Key features and details
- Rabbit polyclonal to NCOR2/SMRT
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-NCOR2/SMRT antibody
See all NCOR2/SMRT primary antibodies -
Description
Rabbit polyclonal to NCOR2/SMRT -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human NCOR2/SMRT aa 1925-1975. The exact sequence is proprietary.
Database link: Q9Y618 -
Positive control
- IP: HeLa cell lysate.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Chromatin Modifying Enzymes
- Acetylation
- HDACs
- Class II / Hda1 Class
Associated products
-
Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab264188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
Use a concentration of 2 - 5 µg/ml.
|
Notes |
---|
IP
Use a concentration of 2 - 5 µg/ml. |
Target
-
Function
Transcriptional corepressor of NR4A2/NURR1 and acts through histone deacetylases (HDACs) to keep promoters of NR4A2/NURR1 target genes in a repressed deacetylated state (By similarity). Mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription. Isoform 1 and isoform 5 have different affinities for different nuclear receptors. -
Tissue specificity
Ubiquitous. High levels of expression are detected in lung, spleen and brain. -
Sequence similarities
Belongs to the N-CoR nuclear receptor corepressors family.
Contains 2 SANT domains. -
Domain
The N-terminal region contains repression functions that are divided into three independent repression domains (RD1, RD2 and RD3). The C-terminal region contains the nuclear receptor-interacting domains that are divided in two separate interaction domains (ID1 and ID2).
The two interaction domains (ID) contain a conserved sequence referred to as the CORNR box. This motif is required and sufficient to permit binding to unligated TR and RARS. Sequences flanking the CORNR box determine nuclear hormone receptor specificity. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 9612 Human
- Omim: 600848 Human
- SwissProt: Q9Y618 Human
- Unigene: 137510 Human
-
Alternative names
- CTG 26 antibody
- CTG repeat protein 26 antibody
- CTG26 antibody
see all
Images
-
NCOR2/SMRT was immunoprecipitated from 1 mg HeLa whole cell lysate using ab264188 at 3 µg/mg lysate. An alternative NCOR2/SMRT antibody was used for western blotting at 1 µg/ml.
Lane 1: Alternative NCOR2/SMRT antibody IP in HeLa whole cell lysate.
Lane 2: ab264188 IP in HeLa whole cell lysate.
Lane 3: Control IgG.Detection: Chemiluminescence with an exposure time of 1 second.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab264188 has not yet been referenced specifically in any publications.