Anti-NCR1 antibody (ab214468)
Key features and details
- Rabbit polyclonal to NCR1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-NCR1 antibody
See all NCR1 primary antibodies -
Description
Rabbit polyclonal to NCR1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human NCR1 aa 150-250 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O76036 -
Positive control
- WB: Mouse spleen and lymph node lysate, and human Raji cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/300.
|
Notes |
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WB
1/300. |
Target
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Function
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. -
Tissue specificity
Selectively expressed by both resting and activated NK cells. -
Sequence similarities
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 2 Ig-like (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated.
O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9437 Human
- Entrez Gene: 17086 Mouse
- Entrez Gene: 117547 Rat
- Omim: 604530 Human
- SwissProt: O76036 Human
- SwissProt: Q8C567 Mouse
- SwissProt: Q9Z0H5 Rat
- Unigene: 97084 Human
see all -
Alternative names
- Activating NK receptor NK p46 antibody
- CD335 antibody
- FLJ99094 antibody
see all
Images
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Anti-NCR1 antibody (ab214468) at 1/300 dilution + Raji cell lysates
Secondary
conjugated secondary antibody at 1/20000 dilutionUpper band could be the result of glycosylation
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All lanes : Anti-NCR1 antibody (ab214468) at 1/1000 dilution
Lane 1 : Mouse lymph node lysate
Lane 2 : Mouse spleen lysate
Secondary
All lanes : Conjugated secondary antibody at 1/20000 dilution
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab214468 has been referenced in 13 publications.
- Tanno L et al. Analysis of Immune Landscape in Pancreatic and Ileal Neuroendocrine Tumours Demonstrates an Immune Cold Tumour Microenvironment. Neuroendocrinology 112:370-383 (2022). PubMed: 34157710
- Synn CB et al. SKI-G-801, an AXL kinase inhibitor, blocks metastasis through inducing anti-tumor immune responses and potentiates anti-PD-1 therapy in mouse cancer models. Clin Transl Immunology 11:e1364 (2022). PubMed: 35003748
- Sodji QH et al. The Combination of Radiotherapy and Complement C3a Inhibition Potentiates Natural Killer cell Functions Against Pancreatic Cancer. Cancer Res Commun 2:725-738 (2022). PubMed: 35937458
- Zhang M et al. SIRT2 promotes murine melanoma progression through natural killer cell inhibition. Sci Rep 11:12988 (2021). PubMed: 34155309
- Li T et al. A Novel C Type CpG Oligodeoxynucleotide Exhibits Immunostimulatory Activity In Vitro and Enhances Antitumor Effect In Vivo. Front Pharmacol 11:8 (2020). PubMed: 32116691