Overview

  • Product name

    Anti-NCR1 antibody [EPR22403-57]
    See all NCR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR22403-57] to NCR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NCR1 aa 1-300. The exact sequence is proprietary.
    Database link: O76036

  • Positive control

    • WB: NK-92 whole cell lysate. IHC-P: Human tonsil and NK/T cell lymphoma.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR22403-57
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab224703 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 34 kDa.
IHC-P 1/1000.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
    • Tissue specificity

      Selectively expressed by both resting and activated NK cells.
    • Sequence similarities

      Belongs to the natural cytotoxicity receptor (NCR) family.
      Contains 2 Ig-like (immunoglobulin-like) domains.
    • Post-translational
      modifications

      N-glycosylated.
      O-glycosylated.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Activating NK receptor NK p46 antibody
      • CD335 antibody
      • FLJ99094 antibody
      • hNKp46 antibody
      • Ly94 antibody
      • Lymphocyte antigen 94 antibody
      • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
      • Lymphocyte antigen 94 homolog antibody
      • Natural cytotoxicity triggering receptor 1 antibody
      • Natural killer cell p46-related protein antibody
      • NCR1 antibody
      • NCT1 antibody
      • NCTR1_HUMAN antibody
      • NK cell activating receptor antibody
      • NK cell-activating receptor antibody
      • NK-p46 antibody
      • NKp46 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human NK/T cell lymphoma tissue labeling NCR1 with ab224703 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human NK/T-cell lymphoma (PMID: 24225754) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    • Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling NCR1 with ab224703 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in marginal zone of human tonsil (PMID: 24225754)  is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    • All lanes : Anti-NCR1 antibody [EPR22403-57] (ab224703) at 1/1000 dilution

      Lane 1 : NK-92 (human malignant non-hodgkin's lymphoma natural killer cell) whole cell lysate
      Lane 2 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 34 kDa
      Observed band size: 35-46 kDa
      why is the actual band size different from the predicted?


      Exposure time: 70 seconds


      The expression profile observed is consistent with what has been described in the literature (PMID:9730896; 9314561).

       Negative control: Raji (PMID: 9730896).

      Blocking/Dilution buffer: 5% NFDM/TBST.

    References

    ab224703 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab224703.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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