Recombinant Anti-NCR1 antibody [EPR22403-57] (ab224703)
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- References
- Protocols
Overview
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Product name
Anti-NCR1 antibody [EPR22403-57]
See all NCR1 primary antibodies -
Description
Rabbit monoclonal [EPR22403-57] to NCR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human NCR1 aa 1-300. The exact sequence is proprietary.
Database link: O76036 -
Positive control
- WB: NK-92 whole cell lysate. IHC-P: Human tonsil and NK/T cell lymphoma.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22403-57 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab224703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 34 kDa. | |
| IHC-P | 1/1000. |
Target
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Function
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. -
Tissue specificity
Selectively expressed by both resting and activated NK cells. -
Sequence similarities
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 2 Ig-like (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated.
O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 9437 Human
- Omim: 604530 Human
- SwissProt: O76036 Human
- Unigene: 97084 Human
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Alternative names
- Activating NK receptor NK p46 antibody
- CD335 antibody
- FLJ99094 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] (ab224703)](https://www.abcam.com/ps/products/224/ab224703/Images/ab224703-2-224703IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] (ab224703)Immunohistochemical analysis of paraffin-embedded human NK/T cell lymphoma tissue labeling NCR1 with ab224703 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in human NK/T-cell lymphoma (PMID: 24225754) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] (ab224703)](https://www.abcam.com/ps/products/224/ab224703/Images/ab224703-1-224703IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR22403-57] (ab224703)Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling NCR1 with ab224703 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining in natural killer (NK) cells in marginal zone of human tonsil (PMID: 24225754) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
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![Western blot - Anti-NCR1 antibody [EPR22403-57] (ab224703)](https://www.abcam.com/ps/products/224/ab224703/Images/ab224703-3-224703WB1.jpg)
Western blot - Anti-NCR1 antibody [EPR22403-57] (ab224703)All lanes : Anti-NCR1 antibody [EPR22403-57] (ab224703) at 1/1000 dilution
Lane 1 : NK-92 (human malignant non-hodgkin's lymphoma natural killer cell) whole cell lysate
Lane 2 : Raji (human burkitt's lymphoma b lymphocyte) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 34 kDa
Observed band size: 35-46 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsThe expression profile observed is consistent with what has been described in the literature (PMID:9730896; 9314561).
Negative control: Raji (PMID: 9730896).
Blocking/Dilution buffer: 5% NFDM/TBST.
Datasheets and documents
References
ab224703 has not yet been referenced specifically in any publications.
