Recombinant Anti-NCR1 antibody [EPR23097-35] (ab233558)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23097-35] to NCR1
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-NCR1 antibody [EPR23097-35]
See all NCR1 primary antibodies -
Description
Rabbit monoclonal [EPR23097-35] to NCR1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IP or WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse spleen and Rat spleen tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23097-35 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (2) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. -
Tissue specificity
Selectively expressed by both resting and activated NK cells. -
Sequence similarities
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 2 Ig-like (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated.
O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 17086 Mouse
- Entrez Gene: 117547 Rat
- SwissProt: Q8C567 Mouse
- SwissProt: Q9Z0H5 Rat
- Unigene: 240231 Mouse
- Unigene: 30049 Rat
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Alternative names
- Activating NK receptor NK p46 antibody
- CD335 antibody
- FLJ99094 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling NCR1 with ab233558 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the rat spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling NCR1 with ab233558 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the mouse spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab233558 has been referenced in 6 publications.
- Rolfes L et al. Natural Killer Cells Are Present in Rag1-/- Mice and Promote Tissue Damage During the Acute Phase of Ischemic Stroke. Transl Stroke Res 13:197-211 (2022). PubMed: 34105078
- Guan X et al. Nanoparticle-enhanced radiotherapy synergizes with PD-L1 blockade to limit post-surgical cancer recurrence and metastasis. Nat Commun 13:2834 (2022). PubMed: 35595770
- Wu J et al. CD147 contributes to SARS-CoV-2-induced pulmonary fibrosis. Signal Transduct Target Ther 7:382 (2022). PubMed: 36424379
- Aliagas E et al. Efficacy of CDK4/6 inhibitors in preclinical models of malignant pleural mesothelioma. Br J Cancer 125:1365-1376 (2021). PubMed: 34588615
- Hu D et al. Grain-sized moxibustion promotes NK cell antitumour immunity by inhibiting adrenergic signalling in non-small cell lung cancer. J Cell Mol Med 25:2900-2908 (2021). PubMed: 33506637
- Hu Q et al. Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma. Cell Death Dis 11:925 (2020). PubMed: 33116117