Recombinant Anti-NCR1 antibody [EPR23097-35] (ab233558)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)](https://www.abcam.com/ps/products/233/ab233558/Images/ab233558-2-anti-ncr1-antibody-epr23097-35-immunohistochemistry-spleen-rat.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23097-35] to NCR1
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-NCR1 antibody [EPR23097-35]
See all NCR1 primary antibodies -
Description
Rabbit monoclonal [EPR23097-35] to NCR1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IP or WB -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse spleen and Rat spleen tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23097-35 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233558 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. -
Tissue specificity
Selectively expressed by both resting and activated NK cells. -
Sequence similarities
Belongs to the natural cytotoxicity receptor (NCR) family.
Contains 2 Ig-like (immunoglobulin-like) domains. -
Post-translational
modificationsN-glycosylated.
O-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 17086 Mouse
- Entrez Gene: 117547 Rat
- SwissProt: Q8C567 Mouse
- SwissProt: Q9Z0H5 Rat
- Unigene: 240231 Mouse
- Unigene: 30049 Rat
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Alternative names
- Activating NK receptor NK p46 antibody
- CD335 antibody
- FLJ99094 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling NCR1 with ab233558 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the rat spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)](https://www.abcam.com/ps/products/233/ab233558/Images/ab233558-1-anti-ncr1-antibody-epr23097-35-immunohistochemistry-spleen-mouse.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling NCR1 with ab233558 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the mouse spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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![Anti-NCR1 antibody [EPR23097-35] (ab233558)](https://www.abcam.com/ps/products/233/ab233558/Images/ab233558-3-benefits-of-recombinant-antibodies.png)
Anti-NCR1 antibody [EPR23097-35] (ab233558)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (2)
ab233558 has been referenced in 2 publications.
- Hu D et al. Grain-sized moxibustion promotes NK cell antitumour immunity by inhibiting adrenergic signalling in non-small cell lung cancer. J Cell Mol Med 25:2900-2908 (2021). PubMed: 33506637
- Hu Q et al. Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma. Cell Death Dis 11:925 (2020). PubMed: 33116117
