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  1. Link

    ncr1-antibody-epr23097-35-ab233558.pdf

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Immunology Adaptive Immunity T Cells Cytotoxic Cells
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RecombinantRabMAb

Recombinant Anti-NCR1 antibody [EPR23097-35] (ab233558)

  • Datasheet
  • Certificate of Compliance
Reviews (2) Submit a question References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)
  • Anti-NCR1 antibody [EPR23097-35] (ab233558)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23097-35] to NCR1
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat

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Overview

  • Product name

    Anti-NCR1 antibody [EPR23097-35]
    See all NCR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR23097-35] to NCR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IP or WB
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse spleen and Rat spleen tissues.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23097-35
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • Cytotoxic Cells
    • Signal Transduction
    • Adapters
    • Transmembrane

Associated products

  • Alternative Versions

    • Anti-NCR1 antibody [EPR23097-35] - BSA and Azide free (ab267792)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab233558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IP or WB.

Target

  • Function

    Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • Tissue specificity

    Selectively expressed by both resting and activated NK cells.
  • Sequence similarities

    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications

    N-glycosylated.
    O-glycosylated.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession O76036 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 17086 Mouse
    • Entrez Gene: 117547 Rat
    • SwissProt: Q8C567 Mouse
    • SwissProt: Q9Z0H5 Rat
    • Unigene: 240231 Mouse
    • Unigene: 30049 Rat
    • Alternative names

      • Activating NK receptor NK p46 antibody
      • CD335 antibody
      • FLJ99094 antibody
      • hNKp46 antibody
      • Ly94 antibody
      • Lymphocyte antigen 94 antibody
      • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
      • Lymphocyte antigen 94 homolog antibody
      • Natural cytotoxicity triggering receptor 1 antibody
      • Natural killer cell p46-related protein antibody
      • NCR1 antibody
      • NCT1 antibody
      • NCTR1_HUMAN antibody
      • NK cell activating receptor antibody
      • NK cell-activating receptor antibody
      • NK-p46 antibody
      • NKp46 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)

      Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling NCR1 with ab233558 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the rat spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NCR1 antibody [EPR23097-35] (ab233558)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling NCR1 with ab233558 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the mouse spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-NCR1 antibody [EPR23097-35] (ab233558)
      Anti-NCR1 antibody [EPR23097-35] (ab233558)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab233558? Please let us know so that we can cite the reference in this datasheet.

    ab233558 has been referenced in 2 publications.

    • Hu D  et al. Grain-sized moxibustion promotes NK cell antitumour immunity by inhibiting adrenergic signalling in non-small cell lung cancer. J Cell Mol Med 25:2900-2908 (2021). PubMed: 33506637
    • Hu Q  et al. Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma. Cell Death Dis 11:925 (2020). PubMed: 33116117

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NCR1 antibody [EPR23097-35]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH9
    Permeabilization
    No
    Specification
    Spleen
    Blocking step
    (agent) for 16 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 4°C
    Fixative
    10% NBF
    Read More

    Vicki Shone

    Verified customer

    Submitted Sep 02 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-NCR1 antibody [EPR23097-35]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (spleen)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
    Permeabilization
    No
    Specification
    spleen
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
    Fixative
    10% NBF
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 24 2020

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