Overview

  • Product name

    Anti-NCR1 antibody [EPR23097-35]
    See all NCR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR23097-35] to NCR1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IP or WB
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Mouse NCR1 aa 150-250. The exact sequence is proprietary.
    Database link: Q8C567

  • Positive control

    • IHC-P: Mouse spleen and Rat spleen tissues.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23097-35
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab233558 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IP or WB.
  • Target

    • Function

      Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
    • Tissue specificity

      Selectively expressed by both resting and activated NK cells.
    • Sequence similarities

      Belongs to the natural cytotoxicity receptor (NCR) family.
      Contains 2 Ig-like (immunoglobulin-like) domains.
    • Post-translational
      modifications

      N-glycosylated.
      O-glycosylated.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Activating NK receptor NK p46 antibody
      • CD335 antibody
      • FLJ99094 antibody
      • hNKp46 antibody
      • Ly94 antibody
      • Lymphocyte antigen 94 antibody
      • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
      • Lymphocyte antigen 94 homolog antibody
      • Natural cytotoxicity triggering receptor 1 antibody
      • Natural killer cell p46-related protein antibody
      • NCR1 antibody
      • NCT1 antibody
      • NCTR1_HUMAN antibody
      • NK cell activating receptor antibody
      • NK cell-activating receptor antibody
      • NK-p46 antibody
      • NKp46 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling NCR1 with ab233558 at 1/500 dilution (0.11 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the rat spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling NCR1 with ab233558 at 1/500 dilution (0.11 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the mouse spleen is observed. The section was incubated with ab233558 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    References

    ab233558 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab233558.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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