Overview

  • Product name

    Anti-NCR1 antibody [MM0491-8F24]
    See all NCR1 primary antibodies
  • Description

    Mouse monoclonal [MM0491-8F24] to NCR1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to the extracellular domain of Human NCR1.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and sterile filtered through a 0.2 µm filter.
  • Clonality

    Monoclonal
  • Clone number

    MM0491-8F24
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 34 kDa.
IHC-Fr 1/20 - 1/100.
Flow Cyt 1/50 - 1/100.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • Tissue specificity

    Selectively expressed by both resting and activated NK cells.
  • Sequence similarities

    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications

    N-glycosylated.
    O-glycosylated.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Activating NK receptor NK p46 antibody
    • CD335 antibody
    • FLJ99094 antibody
    • hNKp46 antibody
    • Ly94 antibody
    • Lymphocyte antigen 94 antibody
    • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
    • Lymphocyte antigen 94 homolog antibody
    • Natural cytotoxicity triggering receptor 1 antibody
    • Natural killer cell p46-related protein antibody
    • NCR1 antibody
    • NCT1 antibody
    • NCTR1_HUMAN antibody
    • NK cell activating receptor antibody
    • NK cell-activating receptor antibody
    • NK-p46 antibody
    • NKp46 antibody
    see all

References

ab89877 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Flow Cytometry
Sample
Human Cell (peripheral blood mononuclear cells (PBMC))
Permeabilization
No
Gating Strategy
lymphocytes based on SSC vs FSC
Specification
peripheral blood mononuclear cells (PBMC)
Fixation
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 14 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Osteosarcoma)
Permeabilization
No
Specification
Osteosarcoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 27°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 25 2015

Application
Flow Cytometry
Sample
Human Cell (PBMCs)
Permeabilization
No
Gating Strategy
Live cell and PBMC population
Specification
PBMCs
Fixation
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 16 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (NK cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
NK cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Abcam user community

Verified customer

Submitted Jun 02 2015

Question
Answer

Thank you for contacting Abcam.

For ab89877, the isotype is IgG2b.

If there is anything else I can help you with, please let me know.

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