Overview

  • Product name
    Anti-NCR1 antibody [n1D9]
    See all NCR1 primary antibodies
  • Description
    Mouse monoclonal [n1D9] to NCR1
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 22-255 of Human NCR1

  • Positive control
    • FC: Human peripheral blood mononuclear cells or natural killer cells, PBMC cells, HeLa cells.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 24/01/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.08% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Ascites
  • Purification notes
    Purified from ascites.
  • Clonality
    Monoclonal
  • Clone number
    n1D9
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14823 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
  • Tissue specificity
    Selectively expressed by both resting and activated NK cells.
  • Sequence similarities
    Belongs to the natural cytotoxicity receptor (NCR) family.
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    N-glycosylated.
    O-glycosylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activating NK receptor NK p46 antibody
    • CD335 antibody
    • FLJ99094 antibody
    • hNKp46 antibody
    • Ly94 antibody
    • Lymphocyte antigen 94 antibody
    • Lymphocyte antigen 94 homolog (activating NK receptor; NK p46) antibody
    • Lymphocyte antigen 94 homolog antibody
    • Natural cytotoxicity triggering receptor 1 antibody
    • Natural killer cell p46-related protein antibody
    • NCR1 antibody
    • NCT1 antibody
    • NCTR1_HUMAN antibody
    • NK cell activating receptor antibody
    • NK cell-activating receptor antibody
    • NK-p46 antibody
    • NKp46 antibody
    see all

Images

  • Flow cytometry analysis of NKp46 in HeLa cells. The cell was stained with ab14823 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

  • Flow cytometry analysis of NKp46 in PBMC cells. The cell was stained with ab14823 at 2-5µg for 1x106 cells (red). A Goat anti mouse IgG (Alexa fluor 488) was used as the secondary antibody. Mouse monoclonal IgG was used as the isotype control (blue), cells without incubation with primary and secondary antibody was used as the negative control (black).

  • ab14823 staining human PBMC by flow cytometry.

    This image was produced using the ascites version of this antibody. 

References

This product has been referenced in:
  • Qu S  et al. Aneustat (OMN54) has aerobic glycolysis-inhibitory activity and also immunomodulatory activity as indicated by a first-generation PDX prostate cancer model. Int J Cancer 143:419-429 (2018). Read more (PubMed: 29441566) »
  • Ascierto ML  et al. Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients. J Transl Med 11:145 (2013). IHC-P . Read more (PubMed: 23758773) »
See all 2 Publications for this product

Customer reviews and Q&As

Answer


Unfortunately, we can not offer a blocking peptide for anti-NCR1 antibody [n1D9] (ab14823), since a recombinant full length protein corresponding to amino acids 22-255 of Human NCR1 was used as immunogen.


I took a look through our catalog and would recommend human NCR1 full length protein (ab132141) or human NCR1 protein fragment (ab191681) for you as it fits your needs. The human NCR1 full length protein (ab132141) compromises the amino acids 22 to 303 and the human NCR1 protein fragment (ab191681) compromises amino acids from 22 to 254. Therefore we would expect either of these products to work with anti-NCR1 antibody [n1D9] (ab14823) as blocking peptide as well as positive control.



You can see the datasheet and extra information here:

https://www.abcam.com/index.html?datasheet=132141 (or use the following: https://www.abcam.com/index.html?datasheet=132141).

https://www.abcam.com/index.html?datasheet=191681 (or use the following: https://www.abcam.com/index.html?datasheet=191681).

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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